Current Position: Research fellow in Comparative Pathology and an Anatomic Pathology Resident at Harvard Medical School
Carole grew up in Rochester, NY and received her undergraduate degree in Biology from Oberlin College in 2000. Her thesis work during her BA program studied the ecology of jack-in-the-pulpit, and she held various research positions studying topics ranging from foot and mouth disease to rattlesnake behavior. After graduation, she worked at Genencor International, a biotechnology company in the San Francisco area, for four years before deciding to pursue veterinary research training. Her career interests include pathology and emerging infectious diseases.
Outside of her research, she enjoys dance (salsa and belly), and yoga, and various outdoor activities such as backpacking and snowboarding.
PhD, Comparative Biomedical Sciences, 2011
The goal of my research is to define requirements in the binding, uptake, and intracellular trafficking of canine and feline parvoviruses during cellular infection. Although CPV and FPV are closely related, there are differences in the binding affinities to canine and feline TfRs, and those affect the host range and infection in different cell types. To probe the consequences of these differences, I am examining the surface attachment and intracellular trafficking patterns of fluorescently-labeled CPV and FPV capsids, with a focus on comparing and contrasting the behavior of different virus strains in cell lines from different species. Live cell microscopy is used to probe the pathways the virus travels through different endosomal compartments.
Publications & Presentations
Markovich JE, Stucker M, Carr AH, Harbison CE, Scarlett JM, Parrish CR. Effects of canine parvovirus strain variations on diagnostic test results and clinical management of enteritis in dogs. J Am Vet Med Assoc. 2012 Jul 1; 241(1):66-72.
Kaelbar JT, Demogines A, Harbison CE, Allison AB, Goodman LB, Ortega AN, Sawyer SL, Parrish CR. Evolutionary Reconstructions of the Transferrin Receptor of Caniforms Supports Canine Parvovirus Being a Re-emerged and Not a Novel Pathogen in Dogs. PLoS Pathog. 2012 May; 8(5):e 1002666.
Cureton DK, Harbison CE, Cocucci E, Parrish CR, Kirchhausen T. Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures. J Virol. 2012 May; 86(9):5330-40.
Harbison CE, Chiorini JA, Parrish CR (in press) The odyssey of the parvovirus capsid - journeying from the cell surface to the nucleus. Trends in Microbiology.
Harbison CE, Weichert WS, Gurda BL, Chiorini JA, Agbandje-McKenna M, Parrish CR. Examining the cross-reactivity and neutralization mechanisms of a panel of mAbs against adeno-associated virus serotypes 1 and 5. J Gen Virol. 2012 Feb; 93(Pt 2):347-55.
Allison AB, Harbison CE, Pagan I, Stucker KM, Kaelber JT, Brown JD, Ruder MG, Keel MK, Dubovi EJ, Holmes EC, Parrish CR. Role of multiple hosts in the cross-species transmission and emergence of a pandemic parvovirus. J Virol. 2012 Jan; 86(2):865-72.
Harbison CE, Lyi SM, Weichert WS, Parrish CR. Early steps in cell infection by parvoviruses: host-specific differences in cell receptor binding but similar endosomal trafficking. J Virol. 2009 Oct; 83(20):10504-14.
Harbison CE, Chiorini JA, Parrish CR. The parvovirus capsid odyssey: from the cell surface to the nucleus. Trends Microbiol. 2008 May; 16(5):208-14.
Chu VC, McElroy LJ, Aronson JM, Oura TJ, Harbison CE, Bauman BE, Whittaker GR. Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus. Virol J. 2007; 4:20.
Doyle, J., Doyle, J., and Harbison, C. 2003. Chloroplast-expressed glutamine synthetase in Glycine and related Leguminosae: Phylogeny, Gene duplication, and Ancient Polyploidy. Systematic Botany. 28 (3): 567-77.
Ferrari, E. Harbison, C. Rashid, H. and Weyler, W. (2003) Enhanced protein expression in Bacillus. Patent # WO 03083125
Xith Parvovirus Workshop, August 2006. "Canine Parvovirus Entry and Trafficking Pathways: Examining requirements for infection through the use of variant receptors." Carole E. Harbison, Erin M. Bennett, Laura S. Palermo, Wendy S. Weichert, and Colin R. Parrish. Poster Presentation
American Society for Virology Conference, July 2007 "Mapping the Entry Pathways of Parvoviruses in Different Cell Types." Carole E. Harbison, Wendy S. Weichert, and Colin R. Parrish, Oral presentation.