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Phone: 607-253-3771
Fax: 607- 253-3071
E-mail: mik7@cornell.edu
Smooth muscle-specific
Cre/eGFP mouse
Light (left) and fluorescent (right) images of mouse urinary bladder (top), mesentery (middle) and heart (below) showing smooth muscle-specific expression of eGFP. (click for larger image)
Dr. Kotlikoff (fifth from right) and the members of his lab.
(click for larger image)
Left column, immunohistochemical staining of GCaMP molecules confined to the smooth muscle layers of the bladder (above), coronary artery (middle), and airway (below). At right the function of the reporter is seen in tissues before and after nerve stimulation of urinary bladder smooth muscle (above), exposure of a mesenteric artery to norepinephrine (middle), and contraction of an airway with methacholine (below). Ca2+ fluorescence is psuedo color-coded from blue to red (see Ji et al, J. Biol. Chem. 279: 21461-21468, 2004).(click for larger image)
Mice with glowing hearts shed light on living cells. |
LAB LINKS
RESEARCH INTERESTS
The Kotlikoff laboratory focuses on the molecular processes underlying excitation-contraction coupling and rhythmicity in cardiac and smooth muscle cells and the processes underlying muscle development. A major goal of the laboratory is to understand processes of intercellular communication and the generation of spontaneous electrical activity. Techniques used to study these processes include gene targeting, development of in vivo imaging methods using genetically targeted cell sensors, embryonic stem cell engraftment, patch clamp measurements of ion channel function, and in vivo imaging. Current projects in the laboratory include the study of the development of electrical activity in the embryonic heart, the use of embryonic cardiomyocytes and embryonic stem cells to alter electrical rhythm disturbances that occur after cardiac injury, the basis of spontaneous rhythm in smooth muscle tissues, and the development of genetically encoded sensors of cell signaling.
EDUCATION
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1973 |
B.A. |
University of Pennsylvania, Philadelphia |
Literature |
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1981 |
V.M.D. |
University of Pennsylvania, Philadelphia |
Vet. Medicine |
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1984 |
Ph.D. |
University of California, Davis |
Physiology |
SELECTED PUBLICATIONS
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Tallini, Y., Ohkura, M., Choi, B.-R., Ji, G., Imoto, K., Doran, R., Lee, J., Plan, P., Wilson, J., Xin, H.-B., Sanbe, A., Gulick, J., Robbins, J., Salama, G., Nakai, J., and Kotlikoff, M.I. Imaging Cellular Signals in the Heart in Vivo: Cardiac Expression of the High Signal Ca2+ Indicator GCaMP2. Proc. Natl. Acad. Sci., U.S.A. 103:4753-4758, 2006.
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Tallini, Y.N., J.F. Brekke, B. Shui, R. Doran, S.M. Hwang, J. Nakai, G. Calama, S.S. Segal, and M.I. Kotlikoff. Propagated endothelial Ca2+ waves and arteriolar dilation in vivo: measurements in Cx40BAC GCaMP2 transgenic mice. Circ. Res. 101:1300-1309, 2007.
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Roell, W., T. Lewalter, B-R Choi, Y.N. Tallini, T. Bostani, U. Becher, P. Sasse, S.-M. Hwang, R. Doran, M. Breitbach, S. Reining, B. Gabris, A. Welz, G. Salama, J.W. Schrickel, M.I. Kotlikoff, and B.K. Fleischmann. Engraftment of connexin -43 expressing cells prevents post -infarct ventricular arrhythmias. Nature 450:819-824, 2007.
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Kolodziejska, KM, HN Ashraf, A Nagy, J Frampton, H-B Xin, MI Kotlikoff, and M Husain. c-Myb –dependent smooth muscle cell differentiation in embryoid bodies. Circ. Res. 102:554-561, 2008.
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Ledoux, J, Taylor, MS, Bonev, AD, Hannah, RM, Solodushko, V, Shui, B. Tallini, Y. Kotlikoff, MI, Nelson, MT. Functional architecture of IP3 signalingin restricted spaces of myoendothelial projections. Proc Natl Acad Sci U S A 105:9627-9632, 2008.
Wang, Q, B Shui, MI Kotlikoff, H Sondermann. Structural basis for calcium sensing by GCaMP2. Structure 16:1817-1827, 2008.
- Tallini, Y, KS Greene, M Craven, A Spealman, M Hesse, J Smith, A Woods, B Singh, A Yen, B Fleischman, and MI Kotlikoff. c-kit+ precursors in the neonatal heart specify all cardiac lineages. Proc Natl Acad Sci. USA 106:1808-1813, 2009.
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