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Biomedical Sciences
Dr. John F. Wootton
Professor of Biochemistry (Emeritus)

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Phone: 607 253 3855
Fax: 607 253 3851
E-mail: jfw1@cornell.edu
MAJOR RESEARCH INTERESTS

My principal interests relate to the functions of proteins interpreted in a broad sense to include fundamental questions regarding relationships between molecular structure/conformation and mechanisms of action (transport, catalysis) and also relationships between molecular properties and roles in physiological processes (protein digestion, absorption and metabolism of nitrogenous compounds, ion transport).

I have studied primarily the proteins involved in intestinal protein digestion (proteases and aminopeptidases), calmodulin and the vitamin D steroid dependent calcium transport system(s) of intestinal mucosal cells, aminotransferases and hemoglobin.

My most recent and ongoing research interest involves the chemical synthesis and characterization of photolabile derivatives of nucleotides and other second messengers and their applications to time-resolved studies of the regulation of metabolism, transport, and signal transduction across biomembranes.

SELECT PUBLICATIONS

  1. Stapleton, S.R., Bell, B.A., Wootton, J.F., and Scott, R.H. (1995) Modulation of Ca2+-dependent currents in metabolically stressed cultured sensory neurons by intracellular photorelease of ATP. British J. Pharmacol. 114: 544-550.
  2. Wootton, J.F., Corrie, J.E.T., Capiod, T., Feeney, J., Trentham, D.R., and Ogden, D.C. (1995) Kinetics of cytosolic Ca2+ concentration after photolytic release of 1-D-myo-inositol-1,4-bisphosphonate-5-phosphorothioate from a caged derivative in guinea pig hepatocytes. Biophysical Journal 68: 2601-2607.
  3. Zhang, B., Wootton, J.F. and Harris-Warrick, R. (1995) Calcium-dependent plateau potentials in a crab stomatogastric ganglion motoneuron. II.calcium-activated slow current. J. Neurophysiol. 74: 1938-1946.
  4. Crawford, J.H., Wootton, J.F., Seabrook, G.R., Scott, R.H. (1997) Activation of Ca2+-dependent currents in dorsal root ganglion neurons by metabolic glutamate receptors and cyclic ADP-ribose precursors. J. Neurophysiol. 77: 2573-2584.
  5. Pollock, J., Crawford, J. H., Wootton, J. F., Seabrook, G. R., and Scott, R. H. (1999) Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones. Cell Calcium 26:139-148.
  6. Eang, L., Corrit, J.E.T., and Wootton, J.F. (2002) Photolabile Precursors of Cyclic Nucleotides with High Aqueous Soluability and Stability. J. Org. Chem. 67: 3474-3478
  7. Pollock, J., Crawford, J.H., Wootton, J.F., Corrie, J.E.T., and Scott, R.H. (2003) A Comparison Between the Distinct Inward Currents Activated in Rat Cultured Dorsal Root Ganglion Neruons by Intracellular Flash Photolysis of Two Forms of Caged Guanosine Monophosphate. Neuroscience Letters 338: 143-146.

For more information, visit my Physiology page.

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