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Patricia Fisher
Director,
Immunopathology
Research
and Development
Laboratory
Department of
Biomedical Sciences
T6-003 VRT
pjf7@cornell.edu
phone: 607 253 3339
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Immunohistochemistry Training
Department of Biomedical Sciences
(1) Contact Pat Fisher for an appointment to discuss your project:
- antibodies you would like to try
- issue types and species, sectioning fixed vs. frozen samples
- project goals, hypothesis, time frame
- scheduling
(2) Training: Day 1
- bring your sections (fixed paraffin embedded, frozen sections, or TC preps)
- supply your primary antibody, all other reagents and controls will be provided
- learn the basics, theory and application, positive and negative controls
- observe and participate in immunostaining protocol (your antibody and samples)
(3) Training: Day 2
- hands on, try the procedure with guidance as needed
(4) On Your Own
- continue trouble shooting your antibody in your own lab, or...
- the Immunopathology Laboratory and reagents are available (see fee schedule)
(5) Cryosectioning
- a cryostat is available in the laboratory for frozen sectioning on a sign-up basis (see fee schedule), this includes instruction in use of the instrument and in methods for freezing samples.
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