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Testing

The FACS sorter is closed until further notice

BD FACS ARIA

The Biomedical Sciences Flow Cytometry Core Laboratory uses a BD-Biosciences FACS Aria high speed flow cytometer/cell sorter utilizing a quartz cuvette for interrogation. It is equipped with three diode lasers providing excitation lines at 488 nm, 633 nm and 407 nm. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. There are 5 emission wavelengths detected from 488 nm excitation, 3 from 633 nm excitation and 3 from 407 nm excitation to accommodate a wide range of fluorochromes. The Aria is capable of high speed cell sorting (>30,000 events/second) and collecting sorted samples into up to four 2ml or 5ml tubes, two 15ml tubes and is also equipped with a robotic device for sorting into multi-well plates and onto glass slides.

FACSARIA

 

 

 

 

 

 

FACSAria Cell Sorter (Current Configuration)
Design of the BD octagon/trigon collection Optics offer greater flexibility than was available in previous instruments. If you need different filter combinations, please ask about different configurations.
Lasers Filters
488nm Argon FITC
515-545nm
PE
564-606nm
PE TEXAS RED
600-620nm
PE/PerCP/Cy-5
675-715nm
PE/Cy-7
750-810nm
633nm HeNe APC
650-670nm
APC/Cy-7
750-810nm
     
407nm Violet Violet1
ALEXA-430
515-545nm
Violet2
DAPI/HOECHST/PacificBlue
430-470nm
     

 

BD LSR II

GUDRUNREITERER

 

 

 

 

 

 

The Biomedical Sciences Flow Cytometry Core Laboratory has an user operated BD-Biosciences LSRII Flow Cytometer also utilizing a quartz cuvette for interrogation. It is equipped with three lasers providing excitation lines at 488nm, 633nm and 407nm, the UV line at 325nm is avalible upon request. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. There are 4 emission wavelengths detected from 488 nm excitation, 2 from 633 nm excitation and 2 from 407 nm excitation to accommodate a wide range of fluorochromes.

 

LSRII (Current Configuration)
Design of the BD octagon/trigon collection Optics offer greater flexibility than was available in previous instruments. If you need different filter combinations, please ask about different configurations.
Lasers Filters
488nm Argon FITC
525/50nm
PI
610/20nm
PE
576/26nm
PE/PerCP/Cy-5
695/40nm
633nm HeNe APC
650-670nm
APC/Cy-7
750-810nm
   
Violet 407nm
Pacific Blue-Alexa 407-DAPI
440/40
Pacific Orange

530/30
   

 

 

 

 


©2014 Cornell University    Last Update January 24, 2014
College of Veterinary Medicine - Ithaca, New York 14853-6401
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