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FACS Sorting
Aria Specs
BD LSR II
Specs
Protocols
Sample prep
Rates
Cytometry Software
Yen Lab
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Cytomtery Resources
All Runs Require Negative unstained controls
(0.5ml minimum volume)
Compensation controls for each fluor
(0.5ml minimum volume)
Acquisition
Samples
It is nice to have 0.5ml of sample volume with 1million cells per ml. Data acquisition runs typically only acquire 10,000 to 30,000 gated events, but still require a minimum 250ul of sample volume.
Sort Runs Cells should be at 20-30 million cells/ml
(5,000 to 35,000 events/s)
The Aria can sort at up to 50- 100 million cells/hour
(a flow rate of 0.2-0.8ul/s and up to 2ul/s with less resolution)
Sort Time ~1-2 ml of sample volume per hour.
Collection rate(cells/hour) = 2(%Viability) X (%Positives) X (cell concentration)
Collection time = (Sort number target) X (collection rate)
* not including set-up
Collection Tubes Investigator should bring collection tubes
1ml, 5ml, or 15ml tubes- some cells require tubes to be coated with BSA/Serum or sorted into tubes containing media
Collection Tubes 1ml microtubes-Bio-Rad-223-9391
5ml (12X75mm)-BD Biosciences-352058
15ml conical-BD Biosciences-352196
Multiwell plates and glass slides
96,48,12,6 well plates max 1.5cm in height
slides should be coated
Questions Please direct all questions to Lavanya at (607)253-4004 biomedflowcore@cornell.edu

Directions

The facility is located in the basement of the Vet. Research Tower down the far right corridor and around the left corner.