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Principal Investigator: Gregory Acland
Contact Information: E-mail: gma2@cornell.edu - Phone: 607-256-5684
Sponsor: Morris Animal Foundation
Grant Number: D07CA-085
Title: Pooled Association Mapping for Canine Hereditary Disorders
Annual Direct Cost: $71,925
Project Period: 09/01/07-08/31/10
DESCRIPTION (provided by applicant): This project will develop a pooled-sample method for association mapping of canine hereditary traits using the Affymetrix Canine SNP Chip. The brlmmp algorithm used to call genotypes from SNP array data will be revised by our collaborators at Broad Institute and tested using DNA from the PI’s laboratory. Once this method is validated, it will be used to map canine hereditary retinal disorders. To test and validate the method, DNA samples will be tested from populations segregating prcd as an autosomal recessive trait, and English Mastiff PRA (T4R Opsin mutation) as an autosomal dominant trait. These populations are selected because the mutation for each is known, and a correct answer can be recognized. Furthermore, in the case of prcd, the extent of linkage disequilibrium surrounding the locus is known, and pools that differ in the extent of their Linkage Disequilibrium (LD) can test the sensitivity and power of the method. The validated pooled-association method will then be used to map the genes associated with colobomas in collie related breeds, cone rod dystrophy 1 in the American Staffordshire Terrier, cone rod dystrophy 2 in the American Pitbull Terrier, cone rod dystrophy 3 in the Irish Glen of Imaal Terrier, and PRA in Italian Greyhounds. Over the 3 year course of the proposed grant several more disorders in addition to these specifically targeted phenotypes will also be mapped.
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