Mentor: Scott Coonrod
Contact Information: Email: email@example.com; Phone: 607-256-5600;
Sponsor: Susan G. Komen Breast Cancer Foundation
Grant Number: KG101303
Title: Role for PAD14 as an Estrogen-Regulated Transcription Cofactor during Breast Cancer
Annual Direct Cost: $60,000
Project Period: 11/10/10- 11/09/13
DESCRIPTION (provided by applicant): Covalent histone modifications such as acetylation, phosphorylation, methylation, and ubiquitination control gene transcription by modulating chromatin structure, and the accessibility of transcription factors to the promoters of specific genes. These modifications are thought to serve as "histone code" to determine the patterns of gene expression. We have found that PADI4, one of peptidylarginine deiminase enzyme family (PADIs) members can convert both unmodified Arg and monomethyl-Arg residues on histones to citrulline. This activity has been correlated with transcriptional repression of a small subset of genes. To broaden our knowledge of the gene regulatory potential of PADI4 (i.e. repressive vs. activating, or both) in breast cancer cells, we have utilized Chromatin immunoprecipitation coupled with microarray (ChIP-chip) analysis to identify the global targets of PADI4.