BD FACS Aria
The Flow Cytometry Core operates a BD FACS Aria high-speed flow cytometer/cell sorter utilizing a quartz cuvette for interrogation. It is equipped with five lasers providing excitation lines at 355 nm, 405 nm, 488 nm, 532 nm and 640 nm. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. The Aria is capable of high speed cell sorting (>30,000 events/second) and collecting sorted samples into up to four 2 ml or 5 ml tubes, two 15 ml tubes and is also equipped with a robotic device for sorting into multi-well plates and onto glass slides.
The Aria also has the following accessories:
- Buffalo Filter Aerosol Management System, which captures any aerosols generated during the sorting process.
- bioBubble Biohazard containment unit which is a negative pressure containment unit that fully encloses the Aria. The bioBubble provides protection for sorting of samples up to BSL2+ particles.
- BD Temperature Control Option, which consists of a recirculating water bath designed to keep collection tube holders at a constant temperature between 4-20 degrees. The recirculating water bath can also be used with the ACDU for temperature-controlled sorting into multi-well plates.
The Aria is only operated by flow core personnel.
BD LSR II
The Biomedical Sciences Flow Cytometry Core Laboratory has a user operated BD-Biosciences LSRII Flow Cytometer also utilizing a quartz cuvette for interrogation. It is equipped with three lasers providing excitation lines at 488nm, 640nm and 407nm. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. There are 6 emission wavelengths detected from 488 nm excitation, 2 from 640 nm excitation and 2 from 407 nm excitation to accommodate a wide range of fluorochromes.
All users must receive training by flow core personnel prior to operating the LSR-II.