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Alerts

  • Updates on testing cattle and cats for Highly Pathogenic Avian Influenza (HPAI)
  • Please note that Platelet Flow Cytometry (PLTFLOW1 & PSAIG) are temporarily unavailable

Canine Respiratory Disease Advisory

 

The Cornell Animal Health Diagnostic Center (AHDC) has recently received multiple reports from veterinary practitioners across the country regarding increased incidence of canine respiratory disease of unknown etiology resulting in pneumonia. Many affected dogs have histories of visiting canine daycare centers, boarding facilities, and dog parks. The reported cases have not responded to normal treatment protocols and experienced a longer, more severe disease course than typical for canine infectious respiratory disease complex. 

Practitioners presented with similar cases are encouraged to perform diagnostic testing immediately, during the acute phase of disease (ideally during the first week of clinical disease) and prior to initiating treatment.  Delaying diagnostics may result in negative test results, as detection of some viruses may be inhibited by a rapid immune response, and antibiotic treatment may impede bacterial growth and detection. The AHDC Veterinary Support Services (VSS) team is available for diagnostic consultation on these cases and can arrange to have appropriate transport media or specimen collection containers overnighted when necessary. Please call (607) 253-3900 or email vetsupport@cornell.edu to reach a VSS veterinarian.

Canine Respiratory Diagnostic Plan

Tests PerformedTest CodeSamples needed
Aerobic Bacterial CultureAER
  • Swab of transtracheal wash (TTW) (preferred sample) or swab of bronchoalveolar lavage (BAL) fluid in Amies transport media or BAL/TTW fluid in red top tube
  • Fresh lung
Anaerobic Bacterial CultureANAER
  • Swab of transtracheal wash (TTW) (preferred sample) or swab of bronchoalveolar lavage (BAL) fluid in anaerobic transport media
  • Fresh lung (>2-3 cm in diameter, minimum size)
  • Swab of lung or piece of lung tissue aseptically collected and immediately placed in anaerobic transport media

Canine Respiratory Panel

*Panel consists of multiple PCR assays:

  • Canine Adenovirus Virus
  • Canine Distemper Virus
  • Canine Parainfluenza Virus
  • Canine Pneumovirus
  • Beta-Coronavirus
  • Bordetella bronchiseptica
  • Mycoplasma Cynos
  • Streptococcus zooepidemicus
  • Influenza A Virus (comprehensive Influenza Virus detection
  • Canine Herpesvirus-1
CRPNL
  • Nasal swab in viral transport media or red top tube with 0.5ml of sterile saline
  • Transtracheal wash (TTW) (preferred sample) or bronchoalveolar lavage (BAL) fluid in red top tube
  • Fresh lung
Fecal FlotationFLOAT
  • Fresh feces in leak-proof container
Fungal CultureFUNGCM
  • Bronchial alveolar lavage (BAL) fluid in red top tube (alternatively can use viral transport media)
  • Transtracheal wash (TTW) (preferred)
  • Fresh lung
Fungal Serology PanelFUNGSER
  • Serum in a red top tube
HistopathologyHISTO
  • Full set of tissues placed in formalin
Mycoplasma Culture

 

MYCOPL

  • Swab of transtracheal wash (TTW) (preferred sample) or swab of bronchoalveolar lavage (BAL) fluid in Amies transport media or BAL/TTW fluid in red top tube
  • Fresh lung
Small Animal HemogramSA  CBC
  • (2) air dried blood smears
  • EDTA whole blood (lavender top tube)
Tracheal Wash CytologyTWE
  • (2) air dried slides of tracheal wash fluid
  • Tracheal wash fluid in EDTA