Development of a Farmer Friendly Molecular Assay for the Detection of Anthelmintic Resistant Stomach Worm (Haemonchus Contortus) of Sheep, Goat, Buffalo and Cows

Principal Investigator: Dwight D. Bowman

Department of Microbiology and Immunology
Sponsor: University of Veterinary and Animal Science- Lahore, Pakistan
Title: Development of a Farmer Friendly Molecular Assay for the Detection of Anthelmintic Resistant Stomach Worm (Haemonchus Contortus) of Sheep, Goat, Buffalo and Cows
Project Amount: $6,860
Project Period: July 2018 to June 2019

DESCRIPTION (provided by applicant): 

The goal is to provide a means of low-cost field sampling of fecal matter for the detection of Haemonchus and resistant Haemonchus in the field under fairly primitive conditions. For this reason, we are targeting the LAMP (Loop Mediated Isothermal Amplification) assay that does not require the use of a thermocycler, and which also has the benefit of being much less susceptible to the presences of reaction inhibitors in feces than other methods. On this project our work will concentrate on assisting in the designs of the test and its application and assistance in the design of the implementation of the testing in the field. Also will be assistance in development of the routine assays with the goal of their having a practical outcome.