As marine ecosystems decline, biodiversity monitoring is vital to detect changes and guide effective conservation. Across the Indo-Pacific Coral Triangle, one of the most biodiverse yet threatened marine ecosystems, climate change, overfishing, and pollution are driving rapid species loss. Many areas remain unassessed due to cost and logistical challenges in remote locations. Our prior work demonstrates the potential of mobile phone–based tools as a practical means of monitoring aquatic food consumption in remote, low-resource settings. This longitudinal data presents a unique opportunity to also serve as a sentinel indicator of biodiversity change and ecosystem use across seasons. By integrating ecological and social science expertise, the team will comprehensively describe aquatic biodiversity and ecosystem use by local communities, and evaluate overlap among ecological data types (environmental DNA, baited remote underwater video systems, and gleaning surveys), and will further examine the extent to which aquatic food consumption data can serve as a sentinel for biodiversity and ecosystem use changes across seasons. The addition of eDNA, BRUVs, and gleaning surveys to a Cornell Atkinson postdoctoral project validitating a mobile phone tool tracking aquatic food consumption will extend this on-going work by providing a comprehensive picture of aquatic biodiversity, including in relation to a newly established MPA, and evaluate the ways high-frequency consumption data may not only be used to assess food security but also for long-term biodiversity and ecosystem use monitoring in hard-to-reach, low-resource settings. The seasonal biodiversity assessment and resulting monitoring tool will support the Timorese Department of Fisheries, WorldFish, and local communities in sustainable monitoring and evidence-based management of marine ecosystems, including traditional management practices. In doing so, this project advances the Cornell K. Lisa Yang Center for Wildlife Health’s mission to transform science into practical policy and action that sustains wildlife, people, and their shared marine environment.