The aim of this project is to establish a robust and reliable immunoassay framework for measuring both total and antigen-specific immunoglobulins (IgA and IgG) in bovine saliva. This includes validating reagents for total bovine Ig quantification. Antibodies against equine IgA will be included for cross-reactivity testing with bovine IgA. Additionally, pending successful reagent validation, a new antigen-specific immunoassay, e.g., ELISA or bead-based assay, will be developed to detect antibodies specific to one or more ArkeaBio-supplied antigens. Together, these tools will support accurate and interpretable immune response analyses in future ArkeaBio field studies.