Principal Investigator: Nicole Buote

DESCRIPTION (provided by applicant): 

Near infrared cholangiography (NIRC) with indocyanine green (ICG) is performed to improve laparoscopic cholecystectomy safety in human patients. This technique has not been reported in clinical veterinary patients, nor has it been used to determine the patency of the biliary tract. This study aims to assess the accuracy of direct NIRC in determining common bile duct (CBD) and major duodenal papilla (MDP) patency. We hypothesize that an injection of ICG into the gallbladder will be visible through the duodenal wall demonstrating biliary system patency. Client-owned dogs and cats (N=20) presenting for gallbladder mucocele or extrahepatic biliary obstruction will be included.

Injection of 2mls of ICG (2.5 mg/ml) into the gallbladder fundus will be performed and intraoperative fluorescence images within the biliary tract and duodenum recorded. Patients will undergo duodenotomy and CBD catheterization to verify patency findings. Fluorescence intensity for duodenal images will be calculated and compared to surgical findings. Patients will receive routine postoperative care including monitoring for any signs of allergic reactions or worsening liver function. Intraoperative and postoperative complications, and mortalities in this population will be reported.

Continuing concerns regarding laparoscopic cholecystectomy center around the inability to accurately detect the biliary system’s patency before removing the gallbladder; however, the risks associated with flushing the common bile duct are not minor and include dehiscence, pancreatitis, and cholangiohepatitis. This procedure could eliminate the need for duodenotomy and flushing regardless of the surgical approach, leading to decreased morbidity and improved outcomes.