Carpal injuries are common in dogs, especially injuries to soft tissue structures that can result in joint instability. Stress radiography is often used to assess for the presence of joint instability and soft tissue injuries. However, there are limited descriptions of standardized acquisition techniques and interpretation guidelines for this imaging procedure. A prior pilot study has established a standardized acquisition technique for carpal stress radiography utilizing a custom-built positioning device and use of a tensionometer to quantify stress forces applied to the joint. The proposed study will utilize this pilot data on canine cadaver limbs to establish carpal joint angle and joint space width ranges that define normal carpal joints and those with disruption of specific ligament groups (collateral ligaments, palmar antebrachiocarpal ligaments, and accessory carpal ligaments). Baseline non-stress radiographs and stress radiographs will be acquired with application of stress forces to the medial joint structures, lateral joint structures, and palmar joint structures, separately. Cadaver limbs will be assigned to one of 8 cohorts. Each cohort will have specific carpal ligament groups transected sequentially, with repeat stress radiographs acquired after transection of each carpal ligament group. Carpal joint angles and joint space width will be measured on all radiographs, with comparisons made between baseline non-stress and stress radiographs, between the ligament groups, and between the cohorts using paired T-tests and repeated measures ANOVA statistical analyses. Study hypotheses are that stress radiography will (1) detect significant differences in carpal joint angles and joint space width between normal carpi and those with induced ligament disruption; and (2) differentiate the type of ligament disruption based on differences in radiographic joint angulation on a series of stress radiograph projections. This will be the first study to objectively quantify differences in radiographic carpal joint angles and joint space widths on stress radiography with injury to specific soft tissue stabilizing structures of the canine carpus.