Bovine Viral Diarrhea

Testing Options for Detection of Individual Bovine Viral Diarrhea Persistently Infected (BVD PI) Animals

Cattle

Antigen Capture ELISA (ACE) on skin samples

  • Any age of cattle can be tested using fresh skin (not fixed)
  • Ear notch samples will not be pooled
  • Lag time = 2-4 days

Antigen Capture ELISA (ACE) on serum samples

  • Cattle must be 61 days of age or older
  • Serum samples will not be pooled for the ACE test
  • Lag time = 2-4 days

Individual PCR

  • Cattle less than 61 days of age – EDTA whole blood required
  • Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
  • Write “Individual PCR” on the submission form
  • Lag time = 3-5 days

Pooled PCR

  • Cattle less than 61 days of age – EDTA whole blood required
  • Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
  • Submit individually labeled samples; pooling is performed at the lab
  • Animals less than 61 days of age will not be pooled with animals 61 days and older
  • Cost per pool (see diagram below for pooling scheme); lag time = 3-5 days
  • Samples from different owners will not be pooled.

Virus Isolation

  • EDTA whole blood for any age but serum is acceptable for animals 61 days and older
  • Lag time = 10-14 day

Camelids

Individual PCR

  • Camelids less than 61 days of age – EDTA whole blood required

  • Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable

  • Write “Individual PCR” on the submission form

  • Lag time = 3-5 days

Pooled PCR

  • Camelids less than 61 days of age – EDTA whole blood required

  • Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable

  • Submit individually labeled samples; pooling is performed at the lab

  • Animals less than 61 days of age will not be pooled with animals 61 days and older

  • Lag time = 3-5 days

  • Samples from different owners will not be pooled

Virus Isolation

  • EDTA whole blood for any age but serum is acceptable for animals 61 days and older

  • Lag time = 10-14 days

Antigen Capture ELISA tests on skin or serum are not valid for camelids.

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