Testing Options for Detection of Individual Bovine Viral Diarrhea Persistently Infected (BVD PI) Animals

Cattle

Antigen Capture ELISA (ACE) on skin samples

• Any age of cattle can be tested using fresh skin (not fixed)
• Ear notch samples will not be pooled
• Lag time = 2-4 days

Antigen Capture ELISA (ACE) on serum samples

• Cattle must be 61 days of age or older
• Serum samples will not be pooled for the ACE test
• Lag time = 2-4 days

Individual PCR

• Cattle less than 61 days of age – EDTA whole blood required
• Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
• Write “Individual PCR” on the submission form
• Lag time = 3-5 days

Pooled PCR

• Cattle less than 61 days of age – EDTA whole blood required
• Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
• Submit individually labeled samples; pooling is performed at the lab
• Animals less than 61 days of age will not be pooled with animals 61 days and older
• Cost per pool (see diagram below for pooling scheme); lag time = 3-5 days
• Samples from different owners will not be pooled.

Virus Isolation

• EDTA whole blood for any age but serum is acceptable for animals 61 days and older
• Lag time = 10-14 day

Camelids

Individual PCR

• Camelids less than 61 days of age – EDTA whole blood required

• Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable

• Write “Individual PCR” on the submission form

• Lag time = 3-5 days

Pooled PCR

• Camelids less than 61 days of age – EDTA whole blood required

• Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable

• Submit individually labeled samples; pooling is performed at the lab

• Animals less than 61 days of age will not be pooled with animals 61 days and older

• Lag time = 3-5 days

• Samples from different owners will not be pooled

Virus Isolation

• EDTA whole blood for any age but serum is acceptable for animals 61 days and older

• Lag time = 10-14 days

Antigen Capture ELISA tests on skin or serum are not valid for camelids.