Bovine Viral Diarrhea
Testing Options for Detection of Individual Bovine Viral Diarrhea Persistently Infected (BVD PI) Animals
Cattle
Antigen Capture ELISA (ACE) on skin samples
- Any age of cattle can be tested using fresh skin (not fixed)
- Ear notch samples will not be pooled
- Lag time = 2-4 days
Antigen Capture ELISA (ACE) on serum samples
- Cattle must be 61 days of age or older
- Serum samples will not be pooled for the ACE test
- Lag time = 2-4 days
Individual PCR
- Cattle less than 61 days of age – EDTA whole blood required
- Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
- Write “Individual PCR” on the submission form
- Lag time = 3-5 days
Pooled PCR
- Cattle less than 61 days of age – EDTA whole blood required
- Cattle 61 days and older – EDTA whole blood, plasma, or serum are accepted
- Submit individually labeled samples; pooling is performed at the lab
- Animals less than 61 days of age will not be pooled with animals 61 days and older
- Cost per pool (see diagram below for pooling scheme); lag time = 3-5 days
- Samples from different owners will not be pooled.
Virus Isolation
- EDTA whole blood for any age but serum is acceptable for animals 61 days and older
- Lag time = 10-14 day
Camelids
Individual PCR
-
Camelids less than 61 days of age – EDTA whole blood required
-
Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable
-
Write “Individual PCR” on the submission form
-
Lag time = 3-5 days
Pooled PCR
-
Camelids less than 61 days of age – EDTA whole blood required
-
Camelids 61 days and older – EDTA whole blood, plasma, or serum are acceptable
-
Submit individually labeled samples; pooling is performed at the lab
-
Animals less than 61 days of age will not be pooled with animals 61 days and older
-
Lag time = 3-5 days
-
Samples from different owners will not be pooled
Virus Isolation
-
EDTA whole blood for any age but serum is acceptable for animals 61 days and older
-
Lag time = 10-14 days
Antigen Capture ELISA tests on skin or serum are not valid for camelids.