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Equine Diagnostic Plans and Panels

The following diagnostic plans have been developed primarily as an educational tool to assist the veterinarian in designing a complete, although not exhaustive, diagnostic strategy. To encourage veterinarians to select only the tests that are deemed clinically appropriate in each situation, the price of the diagnostic plan is not discounted from the sum of the individual test costs.

For the test groupings labeled as panels, there may be a discounted price versus the costs of the individual tests. Diagnostic plans and panels can be requested by writing the complete name as the test request. If appropriate samples are included all tests will be performed.

ACTH and Insulin

Tests Performed Test Code Samples Needed
ACTH & Insulin ACTHIN
  • EDTA plasma (2ml) in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample

Prices

$45.00

Notes

Ship chilled/frozen.

See Equine Cushings test protocol for more information.

ACTH, Insulin and Leptin

Tests Performed Test Code Samples Needed
ACTH, Insulin and Leptin ACINLP
  • EDTA plasma (1.5ml) in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.

Prices

$68.00

Notes

Ship chilled/frozen.

See Equine Metabolic Syndrome Tests and Equine Cushings test protocol and for more information. Also may want to consider Equine Metabolic Diagnostic Plan.

Coagulation Panel, Large Animal

Tests Performed Test Code Samples Needed
Fibrinogen FIB
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
Prothrombin Time PT
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
aPTT (Activated Partial Thromboplastin Time) APTT
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)

Prices

$42.50

Notes

Ship chilled.

Dermatology Diagnostic Plan

All skin biopsy submissions should be accompanied by a Dermatohistopathology Submission Form.

Deep lesion

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included) AER
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
  • +/- Swab from deep in tissue placed in aerobic transport media (Amies with charcoal)
Fungal Culture FUNGCM
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
  • +/- Swab from deep in tissue placed in aerobic transport media (Amies with charcoal)
Gram Stain GRAM
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
Histopathology (biopsy)
  • Multiple formalin fixed skin biopsies.
Parasite Identification PID
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)

Superficial lesion

Tests Performed Test Code Samples Needed
Cytology Smear Exam CYSMR
  • (2) impression smears/skin scrapings/fluid aspirates for cytology placed on glass slides and air dried
Fungal Culture FUNGCM
  • (1) hair and skin scraping for fungal culture and gram stain sent in a sealable paper envelope
Gram Stain GRAM
  • (1) hair and skin scraping for fungal culture and gram stain sent in a sealable paper envelope
Histopathology (biopsy)
  • Multiple formalin fixed skin biopsies.
Skin Scraping SSE
  • (1) skin scraping for ectoparasite identification in an escape proof, nonporous container

Prices

Deep lesion: $233.00
Superficial lesion: $213.00

Testing may qualify for N.Y. State contract case subsidy.

Notes

Deep lesion samples: Ship chilled and protect fixed tissue from freezing.

Superficial lesion samples: Ship at room temperature and protect from temperature extremes. Protect slides from formalin exposure.

DIC Panel, Large Animal

Tests Performed Test Code Samples Needed
Antithrombin AT3
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
D-dimer Quantitative DDIQ
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
Fibrinogen FIB
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
Prothrombin Time PT
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
aPTT (Activated Partial Thromboplastin Time) APTT
  • 2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)

Prices

$103.00

Notes

Ship chilled.

Equine Abortion Fetal Tissue Diagnostic Plan

Tests Performed Test Code Samples Needed
(3) Aerobic bacterial culture (1 sensitivity included) AER
  • Fresh tissue: lung, brain, adrenal, heart, thymus, stomach contents, fetal skin - labelled and individually bagged
Equine Arteritis Virus (EAV) FA EVAFA
  • Fresh tissue: lung, brain, adrenal, heart, thymus, stomach contents, fetal skin - labelled and individually bagged
Equine Arteritis Virus PCR EAR
  • Fresh tissue: placenta, liver, lung, brain, adrenal, heart, thymus, kidney, stomach contents, fetal skin - labelled and individually bagged
Equine Herpesvirus PCR Panel EHVPNL
  • Fresh tissue: placenta, liver, lung, brain, adrenal, heart, thymus, kidney, stomach contents, fetal skin - labelled and individually bagged
Equine Herpesvirus-1 (Rhinopneumonitis) FA EHV1FA
  • Fresh tissue: lung, brain, adrenal, heart, thymus, stomach contents, fetal skin - labelled and individually bagged
Field Necropsy Surcharge HISTONIB
  • Formalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin
Histopathology HISTO
  • Formalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin
(2) Leptospira PCRs LEPTPCR
  • Fresh tissue: lung, brain, adrenal, heart, thymus, stomach contents, fetal skin - labelled and individually bagged
Virus Isolation VI
  • Fresh tissue: lung, brain, adrenal, heart, thymus, stomach contents, fetal skin - labelled and individually bagged
West Nile Virus (WNV) IgG/IgM Capture ELISA Combination WNELPL
  • Fetal heart blood (plain red top tube 1-3ml)
  • Pleural fluid in plain red top tube (1-3ml)

Prices

$575.25 per fetus

Testing may qualify for N.Y. State contract case subsidy.

Notes

Kit is available. See Equine abortion kit for more complete instructions. If full necropsy desired, see Necropsy Testing.

Ship chilled and protect fixed tissue from freezing.

Placenta samples will be cultured as one of the 3 aerobic cultures and held for sufficient time to detect nocardioform.

In abortions from Quarter Horses, Paints and Quarter Horse crosses, testing for glycogen branching enzyme disease should be added. Call for test information. There will be an additional fee for this testing.Formalin fixed tissue6: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, and fetal skin.

Equine Abortion Serology Panel + Selenium

Tests Performed Test Code Samples Needed
Equine Arteritis Virus (EAV) SN EVASN
  • 6ml maternal serum (separated)
Equine Herpesvirus-1 (Rhinopneumonitis) SN EHV1SN
  • 6ml maternal serum (separated)
Equine Influenza Virus (EIV) HAI EIVHAI
  • 6ml maternal serum (separated)
Leptospira MAT, 5 standard serovars LEPTO
  • 6ml maternal serum (separated)
Selenium, whole blood SEL
  • 5ml Maternal EDTA whole blood in lavender top tube

Prices

$164.00 single
$211.00 paired (1 selenium)

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled.

When acute and convalescent samples are/will be collected, for most serology assays they should always be tested in parallel, and paired results interpreted. If acute samples are submitted alone please indicated if all tests should be performed. If second, convalescent samples are later submitted, tests on both samples will be run in parallel and fees for both samples will be charged. To avoid testing acute samples twice, hold and submit with convalescent samples. If convalescent samples will not be collected, or when infectious abortion is strongly suspected due to history, immediate testing of acute sample may be warranted.

Equine Basic Workup

Tests Performed Test Code Samples Needed
Fecal Floatation FLOAT
  • 10g fresh feces from animals 3 days or older
Hemogram (CBC) CBCA
  • (2) air dried blood smears
Large Animal Chemistry LAP
  • 1ml EDTA whole blood in lavender top tube
  • 7ml Serum
Serum Amyloid A SAA
  • 7ml Serum

Prices

$105.00

Notes

Ship chilled except for slides which should remain at room temperature.

Equine Chronic Ill Thrift/Weight Loss Plan

Tests Performed Test Code Samples Needed
ACTH & Insulin ACTHIN
  • 3ml EDTA plasma separated from cells and frozen within 4 hours
ACTH Post TRH ACTHPOSTTRH1
  • 3ml EDTA plasma separated from cells and frozen within 4 hours
ACTH Pre TRH ACTHPRETRH
  • 3ml EDTA plasma separated from cells and frozen within 4 hours
Caseous lumphadenitis RCASE
  • 14ml Serum
Coggins Test EIA
  • 14ml Serum
Fecal Floatation FLOAT
  • 10g fresh feces
Hay Analysis RGEN
  • Hay core sample from 12-20 bales
Hemogram, Large Animal LACBC
  • 2 unstained, unfixed, air-dried blood smears
  • EDTA whole blood (lavender top tube >1/2 full)
Large Animal Chemistry LAP
  • 10ml Serum separated and removed from clot (protected from light and kept cool)
Lawsonia intracellularis IPMA RLAWIPMA
  • 1ml Serum
Lawsonia intracellularis qPCR LAWPCR
  • Feces, illium, or tonsillar tissue
Peritoneal Fluid Cytology PTF
  • 1ml peritoneal fluid in EDTA (PTT); red top tube if culture anticipated plus 2-3 unstained smears.
Sand Recovery Analysis SAND
  • 200g of feces (8-10 fecal balls)
Selenium, whole blood SEL
  • 5ml EDTA whole blood
  • 5ml Heparin whole blood
Serum Amyloid A SAA
  • 14ml Serum
Thymidine Kinase RGEN
  • 5ml Serum
Thyroid Panel, Equine THYPALL
  • 14ml Serum
Vitamin E in serum NDIK
  • 10ml Serum separated and removed from clot (protected from light and kept cool)

Prices

$608.25 to $663.75 depending upon tests ordered

Notes

* Horses with EPM may present with asymmetrical muscle wasting or weakness – diagnostics to consider include Sarcocystis neurona EPM SAG 2,4,3 ELISA and Neospora hughesi ELISA. Both assays are referred to Equine Diagnostic Solutions

* Cyathostomiasis (encysted small strongyle larval infection) may cause chronic ill thrift. In these cases the absence of strongyle eggs and larvae on fecal floatation does not rule out a diagnosis of cyathostomiasis.

* Lawsonia intracellularis, the causative agent of Equine Proliferative Enteropathy, effects horses in the weanling to yearling age range, from approximately 2 to 18 months of age. Rarely adult horses may be affected.

** For diagnosis of PPID if geriatric horses

*** Used to aid in the diagnosis of lymphoma

Equine Complete Ophthalmic Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included) AER
  • Aqueous humour in sterile red top tube (0.2-2.0ml)
  • Conjunctival or corneal swab in Amies transport media
Cytology Smear Exam CYSMR
  • (2) air dried slides of conjunctival or corneal smear
Fungal Culture FUNGCM
  • Conjunctival or corneal swab in Amies transport media
Leptospira MAT, 5 standard serovars LEPTO
  • Serum (separated)
Leptospira PCRs LEPTPCR
  • Aqueous humour in sterile red top tube (0.2-2.0ml)
Lyme Disease (Borrelia burgdorferi) PCR LYMPCR
  • Aqueous humour in sterile red top tube (0.2-2.0ml)
Lyme Disease, Equine Multiplex Assay EQLM
  • Serum (separated)
Parasite Identification PID
  • Conjunctival biopsy (in plain red top tube with 0.5ml saline)

Prices

$320.50

Notes

Ship chilled except for slides should be kept at room temperature.

Equine Cryptorchid Panel

Tests Performed Test Code Samples Needed
Estrone Sulfate E1S
  • 1ml Serum (separated) or plasma
Testosterone baseline TE
  • 1ml Serum (separated) or plasma

Prices

$44.00

Notes

Ship chilled.

Horses must be at least 3 years of age. If less than 3 years consider HCG or GnRH Response, Testosterones:2 and see Large Animal Reproductive Tests for protocols.

Equine Diarrhea, Adult (Acute) Diagnostic Plan

Tests Performed Test Code Samples Needed
Anaerobic bacterial culture ANAER
  • Fecal swab placed in anaerobic transport media
Clostridium difficile Toxins (A/B) CLDT
  • Frozen feces (10 g)
Equine Enteric Coronavirus PCR XEECPCR
  • Fresh feces in leak-proof container (20 g)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (20 g)
Gram Stain GRAM
  • Fresh feces in leak-proof container (20 g)
Hemogram (CBC)
Examination for blood parasites included
CBCA
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated)
Neorickettsia risticii PCR EHRR
  • EDTA whole blood in lavender top tube
Potomac Horse Fever (PHF) IFA PHF
  • Serum (separated)
Salmonella culture (1 sensitivity included) SALM
  • One fecal sample (1 g, fill to line on container but do not overfill)

Prices

$372.00

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled (keep feces for toxin testing frozen) except for ParaPak®, Port-a-cul™ and slides which should be kept at room temperature.

Equine Diarrhea, Adult (Chronic) Diagnostic Plan

Clinical cases of Lawsonia intracellularisare more often documented in horses between 2-18 months of age and rarely in adults.

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included) AER
  • Fresh feces in leak-proof container (230g, 8-10 fecal balls)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (230g, 8-10 fecal balls)
Gram Stain GRAM
  • Fresh feces in leak-proof container (230g, 8-10 fecal balls)
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated)
Lawsonia PCR LAWPCR
  • Fresh feces in leak-proof container (230g, 8-10 fecal balls)
Ref: Lawsonia IPMA RLAWIPMA
  • Serum (separated)
Salmonella culture SALM
  • One fecal sample (1 g, fill to line on container but do not overfill)
Sand Recovery Analysis SAND
  • Fresh feces in leak-proof container (230g, 8-10 fecal balls)
Thymidine Kinase RGEN
  • Serum (separated)

Prices

$434.75

Notes

Ship chilled except for ParaPak® and slides which should be kept at room temperature.

Equine Diarrhea/Septicemia, Neonatal Foal Acute Diarrhea Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (2 sensitivities included)
E. coli and Rhodococcus equi included
AER
  • Fecal swab placed in aerobic transport media (Amies with charcoal)
  • Fresh feces in leak-proof container (20 g)
Anaerobic bacterial culture ANAER
  • Fecal swab placed in anaerobic transport media
(2) Blood Culture, Aerobic BLDAER
  • (2) inoculated aerobic blood culture bottles
(2) Blood Culture, Anaerobic (1 sensitivity included) BLDAN
  • (2) inoculated anaerobic blood culture bottles
Clostridium difficile Toxins (A/B) CLDT
  • Frozen feces (10 g)
Clostridium perfringens Enterotoxin CLPT
  • Frozen feces (10 g)
Equine Enteric Coronavirus PCR XEECPCR
  • Fresh feces in leak-proof container (20 g)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (20 g)
Gram Stain GRAM
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Immunoglobulin G, Equine - TIA EQUINEIGG
  • Serum (separated)
Large Animal Chemistry LAP
  • Serum (separated)
Rotavirus Antigen Detection Group A RLA
  • Fresh feces in leak-proof container (20 g)
Salmonella culture SALM
  • Fecal swab placed in aerobic transport media (Amies with charcoal)
  • Fresh feces in leak-proof container (20 g)
Selenium, whole blood SEL
  • EDTA whole blood in lavender top tube
Serum Amyloid A SAA
  • (2) inoculated aerobic blood culture bottles

Prices

$502.25 (no blood cultures)
$622.25 (w/blood cultures)

Testing may qualify for N.Y. State contract case subsidy.

Notes

If septicemia suspected, include (2) Blood cultures, aerobic and (2) Blood cultures, anaerobic

If fecal float is negative, consider Cryptosporidium ELISA and Giardia ELISA which are performed on fecal samples.

Ship chilled (keep feces for toxin testing frozen) except for blood culture bottles, Port-a-cul™ and slides which should be kept at room temperature.

IgG results available within the same day.

Kit is available. To order blood culture bottles go to shipping services or call 607.253.3935. If non-group A Rotavirus is suspected, Electron Microscopy can be requested.

Equine Fever of Unknown Origin Diagnostic Plan

If case is chronic, virus isolation can be omitted if desired, see comment to right. If not previously performed aLarge animal chemistry panel may be warranted and serum(separated) or heparinized plasma would need to be submitted.

Tests Performed Test Code Samples Needed
(3) Blood Culture, Aerobic/Fungal (1 sensitivity included) BLDAER
  • (3) inoculated aerobic/fungal blood culture bottles
(3) Blood Culture, Anaerobic BLDAN
  • (3) inoculated anaerobic blood culture bottles
Hemogram, Routine
examination for blood parasites included
CBC
  • (2) air dried blood smears
  • (2) tubes EDTA whole blood (lavender top tube)
Serum Amyloid A SAA
  • 5ml Serum
(2) Virus Isolation
nasal, whole blood
VI
  • (2) tubes EDTA whole blood (lavender top tube)
  • Nasal swab in 0.5ml sterile saline to keep moist (not wet)

Prices

$355.00
Chronic case with no virus isolation: $285.00

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled except for blood culture bottles and slides which are shipped at room temperature.

See Blood Culture Technique for more information. To order blood culture bottles go to shipping services or call 607.253.3935.

It may be helpful to collect and bank a serum sample for possible acute and convalescent serology.

This diagnostic plan is designed to detect a broad spectrum of systemic bacterial, viral, and some blood parasite causes of acute fever of unknown origin, as well as certain inflammatory changes which may assist in determining the underlying problems. Viral causes of illness are best detected acutely and most are unlikely to be detected in a patient with a chronic fever of unknown origin This plan is intended to be used as an adjunct to targeted, disease-specific testing appropriate to the species, clinical history and geographic location or conditions. See See Small Animal Internal Medicine, 5th Edition, 2014, Nelson RW and Couto CG; Chapter 88: Fever of Unknown Origin, pages 1279-1282 for other ideas of testing.

Equine Foal Joint Ill Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included)
includes direct plating plus enrichment
AER
  • (1) swab inoculated with synovial fluid and placed in aerobic transport media (Amies)
  • Synovial fluid in EDTA tube (red top tube)
Anaerobic bacterial culture ANAER
  • (1) swab inoculated with synovial fluid and placed in anaerobic transport media
Blood Culture, Aerobic/Fungal BLDAER
  • Inoculated aerobic/fungal blood culture bottle
Blood Culture, Anaerobic BLDAN
  • Inoculated anaerobic blood culture bottle
Hemogram, Routine CBC
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Immunoglobulin G, Equine - Chemistry IGGEQ
  • Serum (separated)
Large Animal Chemistry LAP
  • Serum (separated)
Serum Amyloid A SAA
  • Serum (separated)
Synovial Fluid Cytology
white blood cell count, total protein
JF
  • (2) air dried slides of synovial fluid
  • Synovial fluid in EDTA tube (lavender top tube)

Prices

$383.00

Testing may qualify for N.Y. State contract case subsidy.

Notes

In rare circumstances a Fungal culture may also be warranted.

Synovial fluid can be placed in blood culture bottles to assist in bacterial recovery but sterility of sample procurement is mandatory. Seeblood culture technique for more information. To order blood culture bottles go to shipping services or call (607) 253-3935.

Ship all chilled except for Port-a-cul, blood culture bottles, and slides which should be at room temperature.

Equine Geriatric Horse Diagnostic Plan

Tests Performed Test Code Samples Needed
ACTH & Insulin ACTHIN
  • EDTA plasma in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.
ACTH Post TRH 10min ACTHTRH1
  • EDTA plasma in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.
ACTH Post TRH 30min ACTHTRH2
  • EDTA plasma in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.
ACTH Pre TRH ACTHPRETRH
  • EDTA plasma in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.
Equine Infectious Anemia (EIA) Virus c-ELISA EIAEL
  • Serum (separated, cooled, protected from light)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (10 g)
Hemogram, Routine CBC
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated, cooled, protected from light)
Selenium, whole blood SEL
  • EDTA whole blood in lavender top tube
Vitamin E in serum NDIK
  • Serum (separated, cooled, protected from light)

Prices

$305.00

Notes

Need to include appropriate paperwork for coggins. Ship chilled.

If serum sample is more than 5 days old an EIA AGID will be substituted for the EIA c-ELISA.

Equine Herpesvirus PCR Panel/EHM

Tests Performed Test Code Samples Needed
Equine Herpes Virus 1 Neurological Genotyping EHVN
  • CSF (cerebrospinal fluid) in leak-proof container
  • EDTA whole blood in lavender top tube
  • Nasopharyngeal swab (sterile, sealed vials with several drops of saline)
  • Tissues, min. 2g (lung, liver, spleen, myocardium, placenta, fetus)
  • Tracheal wash (leak-proof container)
Equine Herpes Virus 4 PCR EHV4PCR
  • CSF (cerebrospinal fluid) in leak-proof container
  • EDTA whole blood in lavender top tube
  • Nasopharyngeal swab (sterile, sealed vials with several drops of saline)
  • Tissues, min. 2g (lung, liver, spleen, myocardium, placenta, fetus)
  • Tracheal wash (leak-proof container)
Equine Herpesvirus-1 PCR EHV1PCR
  • CSF (cerebrospinal fluid) in leak-proof container
  • EDTA whole blood in lavender top tube
  • Nasopharyngeal swab (sterile, sealed vials with several drops of saline)
  • Tissues, min. 2g (lung, liver, spleen, myocardium, placenta, fetus)
  • Tracheal wash (leak-proof container)

Prices

$60.00

Notes

Refrigerate and ship chilled.

The Equine Herpesvirus PCR Panel tests for EHV-1 and EHV-4 and includes neurotyping of any EHV-1 positive or suspect samples to assist with diagnosing equine herpesvirus myeloencephalopathy (EHM).

Equine Liver Panel

Plan Code: EQUINE LIVER

Tests Performed Test Code Samples Needed
A/G Ratio
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Albumin ALB
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Aspartate Aminotransferase AST
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Bilirubin Panel (total, direct, indirect) BILIP
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Creatine kinase CK
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Gamma Glutamyl Transferase GGT
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Globulin
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Glutamate Dehydrogenase, blood GLDH
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Sorbitol Dehydrogenase, blood SDH
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Total Protein with Albumin and Globulins
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)
Triglycerides TRIG
  • Heparinized plasma (separated from a green top tube).
  • Serum (separated)

Prices

$25.00

Notes

Ship chilled.

Equine Mare Infertility Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included) AER
  • Uterine swab in Amies transport media
Cytology Smear Exam CYSMR
  • (2) uterine smears air dried slides
Fungal Culture FUNGCM
  • Uterine swab in Amies transport media
Histopathology HISTO
  • Formalin fixed endometrial biopsy
Selenium, whole blood SEL
  • EDTA whole blood in lavender top tube
Vitamin E in serum NDIK
  • Serum (separated, cooled, protected from light)

Prices

$343.50

Notes

May also want to consider (2) Progesterone baseline samples 10-12 days apart or possibly a Granulosa cell panel* (progesterone, testosterone, inhibin included for if indicated. Additional serum would need to be submitted.

Ship culture swabs, EDTA whole blood and serum chilled. Protect fixed tissue from freezing and keep slides at room temperature and protect them from formalin exposure.

Equine Metabolic Syndrome Diagnostic Plan

Tests Performed Test Code Samples Needed
ACTH, Insulin and Leptin ACINLP
  • EDTA plasma in lavender top tube (separated and placed in plastic tube or plain red top tube) and frozen within 4 hours of obtaining sample.
Glucose GLU
  • Serum (separated)
T4 (Thyroxine) - Immulite T4I
  • Serum (separated)

Prices

$94.00

Notes

Ship chilled/frozen. See Equine Metabolic Syndrome Tests for more information.

Equine Myopathy Plan

Horses with EPM may present with asymmetrical muscle wasting or weakness – diagnostics to consider include Sarcocystis neuronaEPM SAG 2,4,3 ELISA and Neospora hughesiELISA. Both assays are referred to Equine Diagnostic Solutions.

Tests Performed Test Code Samples Needed
(2) Creatine kinase CK
  • 1ml Serum or heparinized plasma (GTT) before exercise and after 15 min of walk/trot
Ref: HYPP RHYPP
  • 20-30 hair roots from mane and tail
Ref: Immune Mediated Myositis (MYHM Mutation) RGEN
  • 20-30 hair roots from mane and tail
Ref: Muscle Profile RMUS
  • Semimembranosus or semitendinosus biopsy
Ref: Pollysaccharide Storage RPOLYSB
  • 3-7ml EDTA whole blood or 20 hair roots
Ref: Streptococcus equi ELISA RSTREQ
  • 1-2ml Serum
Selenium, whole blood SEL
  • EDTA or heparin whole blood
Vitamin E in serum NDIK
  • 1ml Serum (separated from clot and protected from light)

Prices

Between $351.00 and $485.00

Notes

MSU: Obtaining a Muscle Sample: Exertional Rhabdomyolysis and PSSM type 1 and PSSM type 2 –Semimembranosus or Semitendinosus; Immune-mediated myositis in QH-related breeds -Fresh semimembranosus and 4-5 fixed Tru Cut samples from epaxial or gluteal muscles

Equine Neurologic Blood Only (Acute) Diagnostic Plan

Rabies should always be considered in neurologic cases. Consult with local health department and AHDC for sample requirements.

Tests Performed Test Code Samples Needed
Equine Herpesvirus-1 PCR EHV1PCR
  • EDTA whole blood in lavender top tube
Ref: EPM SAG 2,3,4 Titer ELISA REPMSAG
  • Serum (separated)
Ref: Encephalitis, Eastern,IgM ELISA REEEIGM
  • Serum (separated)
West Nile Virus (WNV) IgG/IgM Capture ELISA Combination WNELPL
  • Serum (separated)

Prices

$250.50

Notes

May want to retain feed sources including hay for possible botulism testing. Contact lab for more information.

For an increase in sensitivity in diagnosing EHV-1 include a nasal swab in a red top with 0.5 ml sterile saline and request Equine Herpes Virus-1 (EHV-1) PCR for an additional $36.75.

EPM SAG 2,3,4 (CSF: Serum Ratio) is only testing for antibodies to Sarcocystis neurona. If testing for Neospora hughesi please contact lab as the IFA is required.

Note that EEE is not reliably detected in whole blood (CSF and brain tissue are more appropriate samples), however, EEE PCR is warranted on whole blood samples if the season and clinical signs are compatible and other samples are not available.

Equine Neurologic CSF Diagnostic Plan

Rabies should always be considered in neurologic cases. Consult with local health department and AHDC for sample requirements.

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (2) CSF in plain red top tube
Cerebrospinal Fluid Cytology CSF
  • (2) air dried slides of pellet from spun fluid
  • EDTA whole blood in lavender top tube
Equine Herpesvirus PCR Panel EHVPNL
  • (2) CSF in plain red top tube
(2) Equine Herpesvirus-1 PCR EHV1PCR
  • (2) CSF in plain red top tube
  • Nasal swab in 0.5ml sterile saline to keep moist (not wet)
Lyme Disease (Borrelia burgdorferi) PCR LYMPCR
  • (2) CSF in plain red top tube
Lyme Disease, Equine Multiplex Assay EQLM
  • (2) CSF in plain red top tube
  • EDTA whole blood in lavender top tube
Ref: EPM CSF:Serum Ratio REPMSR
  • (2) CSF in plain red top tube
  • Serum (separated)
Virus Isolation VI
  • (2) CSF in plain red top tube
West Nile Virus (WNV) IgG/IgM Capture ELISA Combination WNELPL
  • (2) CSF in plain red top tube
  • Serum (separated)
West Nile Virus (WNV) PCR WNVPCR
  • (2) CSF in plain red top tube

Prices

$580.25

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled except for slides which should be kept at room temperature.

‡ The Equine Herpesvirus PCR Panel tests for EHV-1 and EHV-4 and includes neurotyping of any EHV-1 positive or suspect samples to assist with diagnosing equine herpesvirus myeloencephalopathy (EHM).

EPM SAG 2,3,4 (CSF: Serum Ratio) is only testing for antibodies to Sarcocystis neurona. If testing for Neospora hughesi please contact lab as the IFA is required.

‡‡ Note that EEE is not reliably detected in whole blood (CSF and brain tissue are more appropriate samples), however, EEE PCR is warranted on whole blood samples if the season and clinical signs are compatible and other samples are not available.

Equine Pasture Pregnancy Panel

Tests Performed Test Code Samples Needed
Estrone Sulfate
Estrone sulfate for greater than 90 days pregnant; PMSG for 45-100 days pregnant; Progesterone for less than 60 days pregnant.
E1S
  • Serum (separated)
Pregnant Mare Serum Gonadotropin
Estrone sulfate for greater than 90 days pregnant; PMSG for 45-100 days pregnant; Progesterone for less than 60 days pregnant.
PMSG
  • Serum (separated)
Progesterone - RIA - Equine
Estrone sulfate for greater than 90 days pregnant; PMSG for 45-100 days pregnant; Progesterone for less than 60 days pregnant.
PRE
  • Serum (separated)

Prices

$69.50

Notes

Ship chilled.

Estrone sulfate for greater than 90 days pregnant; PMSG for 45-100 days pregnant; Progesterone for less than 60 days pregnant. See Equine Female Reproductive Testing for more information.

Equine Prepurchase Exam Diagnostic Plan

Tests Performed Test Code Samples Needed
Equine Arteritis Virus (EAV) SN
If stallion, add EVASN
EVASN
  • Serum (separated)
Equine Infectious Anemia (EIA) Virus c-ELISA EIAEL
  • Serum (separated)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (10 g)
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated)
Pre-Purchase Drug Screen PPDS
  • 20ml Heparinized whole blood (green top tube)
  • 5ml of heparinized plasma separated and placed in plain red top tube
  • Urine (50ml) in sterile container

Prices

$264.00
Stallion with EVA SN serology: $280.50

Notes

Ship chilled. Slides should be kept at room temperature.

Need to include appropriate paperwork for Coggins. Do not use gel clot activated tubes (SST). Kit available for sampling, call shipping at (607) 253-3935 to order these.

If stallion, add Equine Arteritis Virus (EAV) SN on serum. If stallion is known EVA positive, may want to consider Equine Arteritis Virus (EAV) semen isolation (OIE) on semen. Contact lab prior to semen collection.

‡ If serum sample is more than 5 days old an EIA AGID will be substituted for the EIA c-ELISA.

Equine Respiratory PCR Panel

Tests Performed Test Code Samples Needed
Equine Adenovirus 1 PCR EAD1
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Equine Adenovirus 2 PCR EAD2
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Equine Arteritis Virus PCR EAR
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Equine Herpes Virus 4 PCR EHV4PCR
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Equine Herpesvirus-1 PCR EHV1PCR
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Equine Rhinitis Virus B PCR ERHB
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Influenza Virus Matrix PCR IVMPCR
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)
Streptococcus equi PCR SEQUPCR
  • Nasal swab or deep pharyngeal swab (in sterile, sealed vials with several drops of saline added)
  • Transtracheal wash or Bronchoalveolar lavage or lung tissue (in sterile leak-proof containers)

Prices

$115.00

Notes

Equine Respiratory Serology Diagnostic Plan

Tests Performed Test Code Samples Needed
Equine Adenovirus (EAD) SN EADSN
  • Serum (separated)
Equine Arteritis Virus (EAV) SN EVASN
  • Serum (separated)
Equine Herpesvirus-1 (Rhinopneumonitis) SN EHV1SN
  • Serum (separated)
Equine Influenza Virus (EIV) HAI EIVHAI
  • Serum (separated)
Equine Rhinitis Virus A PCR ERHA
  • Serum (separated)
Equine Rhinitis Virus B PCR ERHB
  • Serum (separated)

Prices

$91.00 per sample

Testing may qualify for N.Y. State contract case subsidy.

Notes

When acute and convalescent samples are/will be collected, for most serology assays they should always be tested in parallel, and paired results interpreted. If acute samples are submitted alone please indicated if all tests should be performed. If second, convalescent samples are later submitted, tests on both samples will be run in parallel and fees for both samples will be charged. To avoid testing acute samples twice, hold and submit with convalescent samples. If convalescent samples will not be collected, or when infectious abortion is strongly suspected due to history, immediate testing of acute sample may be warranted.

Equine Respiratory, Adult (Acute) Diagnostic Plan

Tests Performed Test Code Samples Needed
Equine Herpesvirus PCR Panel EHVPNL
  • EDTA blood (min. 2ml)
Equine Respiratory Disease Virus Isolation X-refer113
  • Nasal swab in 0.5ml sterile saline to keep moist (not wet)
Equine Respiratory PCR Panel ERPNL
  • Nasal swab in 0.5ml sterile saline to keep moist (not wet)
Streptococcus equi Culture (No sensitivities included) SEQUCUL
  • Nasal swab in aerobic transport media (Amies)

Prices

$267.00

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled. It may be helpful to collect and bank a serum sample for possible acute and convalescent serology.

† This PCR panel tests for equine herpesvirus 1 and 4, equine adenoviruses 1 and 2, equine rhinitis viruses A and B, influenza A virus (matrix) and streptococcus equi. All tests included in this panel are also available individually and expedited results (STAT) are also available upon request for an additional fee. This panel does not include other bacterial respiratory pathogens which would require a transtracheal wash (TTW) or bronchial alveolar lavage (BAL). See chronic respiratory panel below.

†† To maximize sensitivity, it is recommended that BOTH a streptococcus equi PCR (included in the PCR panel) and a streptococcus equi culture be run in parallel (requiring BOTH a nasal swab in a red top tube with 0.5mL of sterile saline and a nasal swab in bacterial transport media).

Equine Respiratory, Adult (Chronic) Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included) AER
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
Anaerobic bacterial culture ANAER
  • (1) swab inoculated with synovial fluid and placed in anaerobic transport media
Equine Herpesvirus 5 qPCR EHV5PCR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Transtracheal wash (TTW) (preferred)
Gram Stain GRAM
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Transtracheal wash (TTW) (preferred)
Tracheal Wash Cytology TW
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Transtracheal wash (TTW) (preferred)

Prices

$204.25

Notes

Ship chilled except for Port-a-cul and slides which should be at room temperature for anaerobes.

See Transtracheal Wash Procedure for more information. [Nasal or pharyngeal swabs are only appropriate for virus isolation and PCR. Submit in viral transport media or a red top tube with a 0.5 ml sterile saline for virus isolation and PCR.

Equine Respiratory, Foal Diagnostic Plan

Tests Performed Test Code Samples Needed
Aerobic bacterial culture (1 sensitivity included)
Rhodococcus equi included
AER
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Transtracheal wash (TTW) (preferred)
Equine Respiratory Disease Virus Isolation X-refer113
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Transtracheal wash (TTW) (preferred)
Equine Respiratory PCR Panel ERPNL
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Transtracheal wash (TTW) (preferred)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (10 g)
Fibrinogen (heat-precipitation) FIB-SCH
  • EDTA whole blood in lavender top tube
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Immunoglobulin G, Equine - TIA EQUINEIGG
  • Serum (separated)
Large Animal Chemistry LAP
  • Serum (separated)
Rhodococcus equi virulence PCR REQUPCR
  • Transtracheal wash (TTW) (preferred)
Tracheal Wash Cytology TW
  • (2) air dried slides of fluid
  • Transtracheal wash (TTW) (preferred)

Prices

$495.75

Testing may qualify for N.Y. State contract case subsidy.

Notes

Ship chilled except for slides which should be at room temperature.

See Transtracheal Wash Procedure for more information. [Nasal or pharyngeal swabs are only appropriate for virus isolation and PCR. Submit in viral transport media or a red top tube with a 0.5 ml sterile saline for virus isolation and PCR.]

†This PCR panel tests for equine herpesvirus 1 and 4, equine adenoviruses 1 and 2, equine rhinitis viruses A and B, influenza A virus (matrix) and streptococcus equi. All tests included in this panel are also available individually and expedited results (STAT) are also available upon request.

§ Rhodococcus equi virulence PCR has only been validated on TTW samples and nasal swabs will be rejected.

Equine Vaccine Titer Plan

Tests Performed Test Code Samples Needed
Equine Herpes Virus 4 SN EHV4SN
  • Serum (separated)
Equine Herpesvirus-1 (Rhinopneumonitis) SN EHV1SN
  • Serum (separated)
Equine Influenza Virus (EIV) HAI EIVHAI
  • Serum (separated)
Equine Rhinitis B Virus SN ERAVSN
  • Serum (separated)
Equine Rhinovirus-1 (ER-A) (Equine Rhinitis A Virus) SN ERAVSN
  • Serum (separated)
Lyme Disease, Equine Multiplex Assay EQLM
  • Serum (separated)
Potomac Horse Fever (PHF) IFA PHF
  • Serum (separated)
Ref: Equine encephalomyelitis PRNT Panel (EEE, WEE, VEE) REWVPRNT
  • Serum (separated)
Ref: Rabies Titer RRABT
  • Serum (separated)
Ref: Streptococcus equi ELISA RSTREQ
  • Serum (separated)
West Nile Virus (WNV) IgG/IgM Capture ELISA Combination WNELPL
  • Serum (separated)

Prices

$429.54

Notes

Ship chilled.

‡ Protective vaccine-induced titers for the various equine diseases have generally not been established. It may be desirable to obtain baseline post-vaccination titers in animals so that later titer testing can be interpreted in light of an animal’s known response to vaccination.

Equine Weanling/Yearling (Acute) Diarrhea Diagnosis

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • Fresh feces in leak-proof container, chilled, not frozen
Anaerobic bacterial culture ANAER
  • Fresh feces in leak-proof container, chilled, not frozen
Antimicrobial susceptibility (systemic) AERSUSC
Clostridium difficile Toxins (A/B) CLDT
  • Fresh feces, frozen
Clostridium perfringens Enterotoxin CLPT
  • Fresh feces, frozen
Coronavirus PCR, Beta BCOR
  • Fresh feces in leak-proof container, chilled, not frozen
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container, chilled, not frozen
Gram Stain GRAM
  • Fresh feces in leak-proof container, chilled, not frozen
Hemogram, Routine CBC
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated)
Lawsonia PCR LAWPCR
  • Feces, illium, or tonsillar tissue
Potomac Horse Fever (PHF) IFA PHF
  • Serum (separated)
Potomac Horse Fever (PHF) PRC
  • EDTA whole blood in lavender top tube
  • Fresh feces in leak-proof container, chilled, not frozen
Ref: Lawsonia IPMA RLAWIPMA
  • Serum (separated)
Rotavirus Antigen Detection Group A RLA
  • Fresh feces in leak-proof container, chilled, not frozen
Salmonella culture SALM
  • Fresh feces in leak-proof container, chilled, not frozen
Sand Recovery Analysis SAND
  • Fresh feces in leak-proof container, chilled, not frozen
Selenium, whole blood SEL
  • EDTA whole blood in lavender top tube

Prices

$597.25

Notes

Equine Wellness Profile Adult/Performance

Tests Performed Test Code Samples Needed
Equine Infectious Anemia (EIA) Virus c-ELISA EIAEL
  • Serum (separated, cooled, protected from light)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (20 g)
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Large Animal Chemistry LAP
  • Serum (separated, cooled, protected from light)
Selenium, whole blood SEL
  • EDTA whole blood in lavender top tube
Serum Amyloid A SAA
  • Serum (separated, cooled, protected from light)
Vitamin E in serum NDIK
  • Serum (separated, cooled, protected from light)

Prices

$218.00

Notes

Ship chilled. Need to include appropriate paperwork for Coggins.

‡ If serum sample is more than 5 days old an EIA AGID will be substituted for the EIA c-ELISA.

Equine Wellness, Newborn Foal (0-48 hours)

Tests Performed Test Code Samples Needed
Hemogram (CBC) CBCA
  • (2) air dried blood smears
  • EDTA whole blood in lavender top tube
Immunoglobulin G, Equine - TIA EQUINEIGG
  • Serum (separated)
Serum Amyloid A SAA
  • Serum (separated)

Prices

$125.00

Notes

Ship chilled except for slides which should be kept at room temperature.

IgG results available within the same day.

Large Animal Renal Panel

Plan Code: LARGE RENAL

Tests Performed Test Code Samples Needed
Albumin ALB
  • 1ml Serum (separated) or heparinized plasma
Anion Gap
  • 1ml Serum (separated) or heparinized plasma
Bicarbonate HCO3FL
  • 1ml Serum (separated) or heparinized plasma
Calcium CA
  • 1ml Serum (separated) or heparinized plasma
Chloride CL
  • 1ml Serum (separated) or heparinized plasma
Creatinine CREAT
  • 1ml Serum (separated) or heparinized plasma
Phosphate (Phosphorus) P
  • 1ml Serum (separated) or heparinized plasma
Potassium K
  • 1ml Serum (separated) or heparinized plasma
Sodium NA
  • 1ml Serum (separated) or heparinized plasma
Urea Nitrogen BUN
  • 1ml Serum (separated) or heparinized plasma

Prices

$28.00

Notes

Ship chilled.

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