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Tests Performed	Test Code	Samples Needed
Fibrinogen	FIB	2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
Prothrombin Time	PT	2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)
aPTT (Activated Partial Thromboplastin Time)	APTT	2ml separated citrate plasma in plastic or siliconized glass tube (blue top tube and then separated and transferred)

Prices

$44.00

Notes

Ship chilled.

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All skin biopsy submissions should be accompanied by a Dermatohistopathology Submission Form.

Prices

Deep lesion: $264.50
Superficial lesion: $305.50

Notes

Deep lesion samples: Ship chilled and protect fixed tissue from freezing.

Superficial lesion samples: Ship at room temperature and protect from temperature extremes. Protect slides from formalin exposure.

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If case is chronic, virus isolation can be omitted if desired. If not previously performed a large animal chemistry panel may be warranted and serum (separated) or heparinized plasma would need to be submitted.

Tests Performed	Test Code	Samples Needed
(3) Blood Culture, Aerobic	BLDAER	(3) inoculated aerobic/fungal blood culture bottles
(3) Blood Culture, Anaerobic	BLDAN	(3) inoculated anaerobic blood culture bottles
Fibrinogen (heat-precipitation)	FIB-SCH	(2) tubes EDTA whole blood (lavender top tube)
Hemogram (CBC), Automated examination for blood parasites included	CBCA	(2) air dried blood smears (2) tubes EDTA whole blood (lavender top tube)
(2) Virus Isolation nasal, whole blood	VI	(2) tubes EDTA whole blood (lavender top tube) Nasal swab in 0.5ml sterile saline to keep moist (not wet)

Prices

$327.00
Chronic case with no virus isolation: $252.00

Notes

Ship chilled except for blood culture bottles and slides which are shipped at room temperature.

See Blood Culture Technique for more information. To order blood culture bottles go to shipping services or call 607.253.3935.

It may be helpful to collect and bank a serum sample for possible acute and convalescent serology.

This diagnostic plan is designed to detect a broad spectrum of systemic bacterial, viral, and some blood parasite causes of acute fever of unknown origin, as well as certain inflammatory changes which may assist in determining the underlying problems. Viral causes of illness are best detected acutely and most are unlikely to be detected in a patient with a chronic fever of unknown origin. This plan is intended to be used as an adjunct to targeted, disease-specific testing appropriate to the species, clinical history and geographic location or conditions. See Small Animal Internal Medicine, 5th Edition, 2014, Nelson RW and Couto CG; Chapter 88: Fever of Unknown Origin, pages 1279-1282 for other ideas of testing.

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Tests Performed	Test Code	Samples Needed
Fibrinogen (heat-precipitation)	FIB-SCH	EDTA whole blood in lavender top tube
Hemogram (CBC), Automated	CBCA	(2) air dried blood smears EDTA whole blood in lavender top tube
Large Animal Chemistry	LA P	Heparinized plasma (separated from a green top tube). Serum (separated)

Prices

$110.00

Notes

Ship chilled.

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Tests Performed	Test Code	Samples Needed
A/G Ratio		Heparinized plasma (separated from a green top tube). Serum (separated)
Albumin	ALB	Heparinized plasma (separated from a green top tube). Serum (separated)
Aspartate Aminotransferase	AST	Heparinized plasma (separated from a green top tube). Serum (separated)
Bilirubin Panel (total, direct, indirect)	BILIP	Heparinized plasma (separated from a green top tube). Serum (separated)
Creatine kinase	CK	Heparinized plasma (separated from a green top tube). Serum (separated)
Gamma Glutamyl Transferase	GGT	Heparinized plasma (separated from a green top tube). Serum (separated)
Globulin		Heparinized plasma (separated from a green top tube). Serum (separated)
Glutamate Dehydrogenase, blood	GLDH	Heparinized plasma (separated from a green top tube). Serum (separated)
Sorbitol Dehydrogenase, blood	SDH	Heparinized plasma (separated from a green top tube). Serum (separated)
Total Protein with Albumin and Globulins		Heparinized plasma (separated from a green top tube). Serum (separated)
Triglycerides	TRIG	Heparinized plasma (separated from a green top tube). Serum (separated)

Prices

$33.00

Notes

Ship chilled.

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Plan Code: LARGE RENAL

Tests Performed	Test Code	Samples Needed
Albumin	ALB	1ml Serum (separated) or heparinized plasma
Anion Gap		1ml Serum (separated) or heparinized plasma
Bicarbonate	HCO3FL	1ml Serum (separated) or heparinized plasma
Calcium	CA	1ml Serum (separated) or heparinized plasma
Chloride	CL	1ml Serum (separated) or heparinized plasma
Creatinine	CREAT	1ml Serum (separated) or heparinized plasma
Phosphate (Phosphorus)	P	1ml Serum (separated) or heparinized plasma
Potassium	K	1ml Serum (separated) or heparinized plasma
Sodium	NA	1ml Serum (separated) or heparinized plasma
Urea Nitrogen	BUN	1ml Serum (separated) or heparinized plasma

Prices

$29.00

Notes

Ship chilled.

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Tests Performed	Test Code	Samples Needed
(3) Aerobic bacterial culture Listeria included	AER	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Bovine Virus Diarrhea Virus (BVDV) FA	BVDFA	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Bovine Virus Diarrhea Virus (BVDV) SN, Type 1	BVDSN	Heart blood (serum), peritoneal, pleural, or pericardial fluid in sterile red top tube.
Bovine Virus Diarrhea Virus (BVDV) SN, Type 2	BVDSN2	Heart blood (serum), peritoneal, pleural, or pericardial fluid in sterile red top tube.
(2) Campylobacter fetus (Vibrio) FA	CAMPYFA	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Campylobacter jejuni Culture	CAMPYJ	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Chlamydophila FA	CHFA	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Chlamydophila PCR	CHPCR	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Coxiella burnetii PCR	COXPCR	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Field Necropsy Surcharge	HISTONIB	Formalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin
Histopathology	HISTO	Formalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin
Leptospira PCR	LEPTPCR	Placenta/cotyledon. If not available, fetal lung, stomach or abomasal content
Leptospira PCR	LEPTPCR	Fetal kidney. If not available, fetal lung, stomach or abomasal content
Toxoplasma gondii lgG ELISA	TOXEL	Heart blood (serum), peritoneal, pleural, or pericardial fluid in sterile red top tube.
Toxoplasmosis FA	TOXFA	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin
Virus Isolation	VI	Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin

Prices

$702.00 per fetus

Notes

Ship fresh tissue chilled and protect fixed tissue from freezing. Kit is available. See ruminant abortion kit for more complete instructions. If full necropsy desired, see necropsy testing.

Consider adding Cache Valley Fever SN to fetal heart blood (serum), peritoneal, pleural, or pericardial fluid if the first trimester of the pregnancy corresponds to a period of time in which there is mosquito activity in your geographic location.

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Tests Performed	Test Code	Samples Needed
Bovine Virus Diarrhea Virus (BVDV) SN, Type 1	BVDSN	Maternal serum (separated)
Bovine Virus Diarrhea Virus (BVDV) SN, Type 2	BVDSN2	Maternal serum (separated)
Chlamydophila CF	CHCF	Maternal serum (separated)
Coxiella burnettii ELISA	COXEL	Maternal serum (separated)
Leptospira MAT, 5 standard serovars	LEPTO	Maternal serum (separated)
Selenium, whole blood	SEL	Maternal EDTA whole blood in lavender top tube
Toxoplasma gondii lgG ELISA	TOXEL	Maternal serum (separated)

Prices

$125.50 single
$196.50 paired (1 selenium)

Notes

Ship chilled.

When acute and convalescent samples are/will be collected, for most serology assays they should always be tested in parallel, and paired results interpreted. If acute samples are submitted alone please indicated if all tests should be performed. If second, convalescent samples are later submitted, tests on both samples will be run in parallel and fees for both samples will be charged. To avoid testing acute samples twice, hold and submit with convalescent samples. If convalescent samples will not be collected, or when infectious abortion is strongly suspected due to history, immediate testing of acute sample may be warranted.

If history indicates may also want toconsider Brucella ovis ELISA* which isperformed on serum

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Tests Performed	Test Code	Samples Needed
Fecal Floatation	FLOAT	Fresh feces in leak-proof container (20 g)
Johne’s Commercial ELISA		Serum (separated)
Salmonella culture	SALM	Fresh feces in leak-proof container (20 g) One fecal sample (1 g, fill to line on container but do not overfill) can be put in ParaPak® transport media to assist with Salmonella recovery

Prices

$55.00

Notes

Ship chilled, except for ParaPak® which should be kept at room temperature.

Johne's Culture, Non-Bovine on fresh feces may also be warranted. The lag time for culture is 12-16 weeks although positive samples may be shorter. In neonatal lambs, an Aerobic bacterial culture and a Rotavirus Latex Antigen Detection, Group A on feces should also be considered.

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Tests Performed	Test Code	Samples Needed
Aerobic bacterial culture	AER	(1) swab inoculated with CSF (1) swab of meninges prior to contamination and placed in aerobic transport media (Amies with charcoal) CSF sample taken using sterile technique and placed in sterile red top tube
Eastern Equine Encephalitis Virus PCR	EEEPCR	Brain or spinal cord CSF EDTA Whole Blood
Field Necropsy Surcharge	HISTONIB
Histopathology	HISTO	Full set of fixed tissues placed in formalin, including multiple sections of brain and spinal cord.
Lead (blood)	BDLEAD	EDTA or Heparin whole blood (lavender or green top tube) or fresh liver in sterile container Fresh liver in sterile container
Lead (tissue)	NCOX	EDTA or Heparin whole blood (lavender or green top tube) or fresh liver in sterile container Fresh liver in sterile container
Listeria Culture	LISTCUL	Fresh brain (preferably brainstem) placed in sterile container
Ref: Equine encephalomyelitis PRNT Panel (EEE, WEE, VEE) Referral Fee - $30.00	REWVPRNT	2 ml Serum
West Nile Virus (WNV) PCR	WNVPCR	CSF, brain or spinal cord
West Nile Virus (WNV) SN	WNVSN	Serum, acute and convalescent

Prices

$439.00

Notes

Ship chilled and protect fixed tissue from freezing. Please indicate if rabies testing is pending.

*Consider mosquito-borne virus testing based on compatible clinical signs, season, and geographic location.

**Note that EEE is not reliably detected in EDTA whole blood (CSF and brain tissue are preferred), however EEE PCR is warranted on EDTA whole blood if other preferred samples are not available.

See Tissues for Rabies Diagnosis for photos of brain samples needed for rabies testing.

May also want to consider Cerebrospinal Fluid Cytology which requires CSF in EDTA tube (LTT) and (2) air dried slides of CSF. Protect slides from formalin exposure.

If whole head is submitted a rabies brain extraction fee will be applied. Please submit completed NYS rabies specimen history form and write out clear instructions on what additional diagnostic tests should be performed.

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Prices

$358.00 (ante mortem samples)
$370.00 (full set of necropsy samples)

Notes

Testing for evidence of lungworms, Fecal Float may be warranted depending on season. Also selenium on an EDTA whole blood sample or liver (Selenium, tissue) and vitamin E on a serum sample (separated) may be appropriate in some situations. Limited serology testing is available but paired acute and convalescent serum samples for BRSV SN may be warranted in acute disease episodes.

Ante mortem: Ship chilled except for slides which should be kept at room temperature.

Necropsy: Ship fresh tissue and swab chilled and protect fixed tissue from freezing.

TTW fluid is a superior sample for detection of significant bacterial pathogens causing lower airway disease. Results of nasal or pharyngeal swab cultures must be interpreted with caution as many bacterial organisms may be present as normal commensals in the upper respiratory tract. See Transtracheal Wash Procedure for more information. It may be helpful to collect and bank a serum sample for possible acute and convalescent serology.

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Tests Performed	Test Code	Samples Needed
Bovine Virus Diarrhea Virus (BVDV) PCR	BVDPCR	EDTA whole blood in lavender top tube
Fecal Floatation	FLOAT	Fresh feces in leak-proof container (20 g)
Fibrinogen (heat-precipitation)	FIB-SCH	EDTA blood (tube >1/2 full)
Hemogram, Routine	LA CBC	EDTA whole blood in lavender top tube
Johne's AGID	JAGID	Serum (separated)
Johne's Fecal Culture	JCNB	Fresh feces in leak-proof container (20 g)
Large Animal Chemistry	LA P	EDTA whole blood in lavender top tube Heparinized plasma (separated from a green top tube). Serum (separated)
Selenium, whole blood	SEL	EDTA whole blood in lavender top tube Serum (separated)
Small Ruminant Lentivirus cELISA	SRLEL	Serum (separated)
Vitamin E in serum	NDIK	Serum (separated)

Prices

$294.75

Notes

* Haemonchus ID is in investigational mode, but should be validated soon. This test is available currently if requested. There must be >100 Strongyle EPG to perform this test. This will incur an additional charge.

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Tests Performed	Test Code	Samples Needed
Aerobic bacterial culture	AER	Conjunctival swab in aerobic transport media (Amies without charcoal)
Chlamydophila PCR	CHPCR	Conjunctival swab in sterile tube with 0.5ml saline to keep moist (not wet).
Mycoplasma Culture	MYCOPL	Conjunctival swab in aerobic transport media (Amies without charcoal)

Prices

$108.00

Notes

Ship chilled.