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Porcine Diagnostic Plans and Panels

Updated July 12, 2019

The following diagnostic plans have been developed primarily as an educational tool to assist the veterinarian in designing a complete, although not exhaustive, diagnostic strategy. To encourage veterinarians to select only the tests that are deemed clinically appropriate in each situation, the price of the diagnostic plan is not discounted from the sum of the individual test costs.

For the test groupings labeled as panels, there may be a discounted price versus the costs of the individual tests. Diagnostic plans and panels can be requested by writing the complete name as the test request. If appropriate samples are included all tests will be performed.

Antimicrobial susceptibilities for culture isolates are available upon request at an additional charge.

Print Plans and Panels

Dermatology Diagnostic Plan

All skin biopsy submissions should be accompanied by a Dermatohistopathology Submission Form.

Deep lesion

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
  • +/- Swab from deep in tissue placed in aerobic transport media (Amies with charcoal)
Fungal Culture FUNGCM
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
  • +/- Swab from deep in tissue placed in aerobic transport media (Amies with charcoal)
Gram Stain GRAM
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)
Histopathology (biopsy) HISTO
  • Multiple formalin fixed skin biopsies.
Parasite Identification PID
  • (2) fresh skin biopsies in plain red top tube with 0.5ml sterile saline to keep moist (not wet)

Superficial lesion

Tests Performed Test Code Samples Needed
Cytology Smear Exam CYSMRE
  • (2) impression smears/skin scrapings/fluid aspirates for cytology placed on glass slides and air dried
Fungal Culture FUNGCM
  • (1) hair and skin scraping for fungal culture and gram stain sent in a sealable paper envelope
Gram Stain GRAM
  • (1) hair and skin scraping for fungal culture and gram stain sent in a sealable paper envelope
Histopathology (biopsy) HISTO
  • Multiple formalin fixed skin biopsies.
Skin Scraping SSE
  • (1) skin scraping for ectoparasite identification in an escape proof, nonporous container

Prices

Deep lesion: $264.50
Superficial lesion: $305.50

Notes

Deep lesion samples: Ship chilled and protect fixed tissue from freezing.

Superficial lesion samples: Ship at room temperature and protect from temperature extremes. Protect slides from formalin exposure.

Porcine Abortion Fetal Tissue Diagnostic Plan

Please note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia and SIV. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Tests Performed Test Code Samples Needed
(3) Aerobic bacterial culture
Listeria included
AER
  • Fresh tissue: lung, thoracic fluid, placenta, stomach contents, kidney, liver, heart, spleen, lymph nodes, adrenal, skin, intestine, heart blood in sterile red top tube
Field Necropsy Surcharge HISTONIB
  • Formalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin, and umbilicus.
Histopathology HISTO
Leptospira PCR LEPTPCR
  • Placenta/cotyledon. If not available, fetal lung, stomach or abomasal content
Leptospira PCR LEPTPCR
  • Fetal kidney. If not available, fetal lung, stomach or abomasal content
Porcine Circovirus type 2 (PCV2) FA VIRFA
  • Fresh tissue: lung, thoracic fluid, placenta, stomach contents, kidney, liver, heart, spleen, lymph nodes, adrenal, skin, intestine, heart blood in sterile red top tube
Porcine Parvovirus FA VIRFA
  • Fresh tissue: lung, thoracic fluid, placenta, stomach contents, kidney, liver, heart, spleen, lymph nodes, adrenal, skin, intestine, heart blood in sterile red top tube
Porcine Reproductive and Respiratory Syndrome Virus FA (PRRSV) VIRFA
  • Fresh tissue: lung, thoracic fluid, placenta, stomach contents, kidney, liver, heart, spleen, lymph nodes, adrenal, skin, intestine, heart blood in sterile red top tube
Virus Isolation VI
  • Fresh tissue: lung, thoracic fluid, placenta, stomach contents, kidney, liver, heart, spleen, lymph nodes, adrenal, skin, intestine, heart blood in sterile red top tube

Prices

$516.00 per fetus

Notes

Ship fresh tissue chilled and protect fixed tissue from freezing.

For cases with mummified fetuses you may also want to consider Porcine Parvovirus HI and Porcine Circovirus-2 IFA on fetal heart blood. For comprehensive rule out of PRRSV, the PCR is recommended on lung and/or thoracic fluid. If abortion is suspected to be related to ventilation then carbon monoxide testing on heart blood in an EDTA tube or a red top or thoracic fluid is recommended.

Porcine Diarrhea, Nursing Pigs Diagnostic Plan

PLEASE NOTE THAT ACCURATE DIAGNOSIS OF DIARRHEA IN NURSING PIGS USUALLY REQUIRES SUBMISSION OF TISSUES. Results of tests on feces or fecal swabs only, must be evaluated with consideration of clinical signs. Accurate diagnosis requires the detection of the agent(s) as well as compatible histologic lesions from post mortem samples.

Please also note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia and SIV. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Ante mortem

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • Fecal swab placed in anaerobic transport media
  • Fresh feces in leak-proof container (20 g)
Alpha Coronavirus (TGEV) PCR ACOR
  • Fresh feces in leak-proof container (20 g)
Anaerobic bacterial culture ANAER
  • Fecal swab placed in anaerobic transport media
Clostridium difficile Toxins (A/B) CLDT
  • Frozen feces (5 g)
Clostridium perfringens Enterotoxin CLPT
  • Frozen feces (5 g)
Fecal Floatation
(if ≥ 3 days old)
FLOAT
  • Fresh feces in leak-proof container (20 g)
Porcine Delta Coronavirus PCR* RPDCPCR
  • Fresh feces in leak-proof container (20 g)
Porcine Epidemic Diarrhea Virus (PEDV) PCR* RPEDPCR
  • Fresh feces in leak-proof container (20 g)
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Serum or Oral fluid
Rotavirus Antigen Detection Group A RLA
  • Fresh feces in leak-proof container (20 g)
Salmonella culture SALM
  • Fecal swab placed in anaerobic transport media
  • Fresh feces in leak-proof container (20 g)
  • One fecal sample (1 g, fill to line on container but do not overfill) can be put in ParaPak® transport media to assist with Salmonella recovery

Necropsy

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Alpha Coronavirus (TGEV) PCR ACOR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Anaerobic bacterial culture ANAER
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (frozen)
Clostridium difficile Toxins (A/B) CLDT
  • Tied off loop of bowel with contents (frozen)
Clostridium perfringens Enterotoxin CLPT
  • Tied off loop of bowel with contents (frozen)
Fecal Floatation
(if ≥ 3 days old)
FLOAT
  • Fresh feces or colon contents in leak-proof container (10 g)
Field Necropsy Surcharge HISTONIB
  • Full set of formalin fixed tissues (including multiple sections of intestinal tract)
Histopathology HISTO
  • Full set of formalin fixed tissues (including multiple sections of intestinal tract)
Porcine Delta Coronavirus PCR* RPDCPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Epidemic Diarrhea Virus (PEDV) PCR* RPEDPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Fresh tissues: lung, lymph nodes, tonsils, and spleen
Salmonella culture SALM
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
(2) Virus FA
Porcine Rotavirus, PRRSV
VIRFA
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Virus Isolation VI
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)

Prices

$485.80 (ante mortem samples)
$687.00 (full set of necropsy samples)

Notes

* Consider the PED/TGE combination PCR test. May want to consider Clostridium perfringens genotyping PCR for identification of the genes for specific toxins if Clostridium perfringens is isolated. If E. coli is isolated, consider Escherichia coli genotyping PCR to further identify which genes for which adhesins and toxins are present.

Ship chilled and keep frozen samples frozen except for Port-a-cul™ or Anaerobic Systems ATM and Parapak® which should be kept at room temperature. Protect fixed tissue from freezing.

Porcine Diarrhea, Weaned Pigs Diagnostic Plan

PLEASE NOTE THAT ACCURATE DIAGNOSIS OF DIARRHEA IN WEANED PIGS USUALLY REQUIRES SUBMISSION OF TISSUES. Results of tests on feces or fecal swabs only, must be evaluated with consideration of clinical signs. Accurate diagnosis requires the detection of the agent(s) as well as compatible histologic lesions from post mortem samples.

Please also note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia, SIV, and PED. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Ante mortem

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • Fecal swab placed in anaerobic transport media
  • Fresh feces in leak-proof container (20 g)
Alpha Coronavirus (TGEV) PCR ACOR
  • Fresh feces in leak-proof container (20 g)
Anaerobic bacterial culture ANAER
  • Fecal swab placed in anaerobic transport media
Brachyspira culture* RBRACH
  • Fresh feces in leak-proof container (20 g)
  • Rectal swab (preferred ante mortem sample for Brachyspira) placed in aerobic transport media (Amies with charcoal) or anaerobic transport media
Clostridium perfringens Enterotoxin CLPT
  • Frozen feces (5 g)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (20 g)
Lawsonia PCR LAWPCR
  • Fresh feces in leak-proof container (20 g)
Porcine Delta Coronavirus PCR* RPDCPCR
  • Fresh feces in leak-proof container (20 g)
Porcine Epidemic Diarrhea Virus (PEDV) PCR* RPEDPCR
  • Fresh feces in leak-proof container (20 g)
  • Oral fluid
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Serum or Oral fluid
Rotavirus Antigen Detection Group A RLA
  • Fresh feces in leak-proof container (20 g)
Salmonella culture SALM
  • Fecal swab placed in anaerobic transport media
  • Fresh feces in leak-proof container (20 g)
  • One fecal sample (1 g, fill to line on container but do not overfill) can be put in ParaPak® transport media to assist with Salmonella recovery
Yersinia culture YERS
  • Fecal swab placed in anaerobic transport media
  • Fresh feces in leak-proof container (20 g)
  • One fecal sample (1 g, fill to line on container but do not overfill) can be put in ParaPak® transport media to assist with Salmonella recovery

Necropsy

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Alpha Coronavirus (TGEV) PCR ACOR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Anaerobic bacterial culture ANAER
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (frozen)
Brachyspira culture* RBRACH
  • Colon or cecum (preferred for Brachyspira)
  • Tied off loop of bowel with contents (fresh)
Clostridium perfringens Enterotoxin CLPT
  • Tied off loop of bowel with contents (frozen)
Fecal Floatation FLOAT
  • Fresh feces in leak-proof container (10 g)
Field Necropsy Surcharge HISTONIB
  • Full set of formalin fixed tissues (including multiple sections of intestinal tract)
Histopathology HISTO
  • Full set of formalin fixed tissues (including multiple sections of intestinal tract)
Lawsonia PCR LAWPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Delta Coronavirus PCR* RPDCPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Epidemic Diarrhea Virus (PEDV) PCR* RPEDPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Fresh tissues: lung, lymph nodes, tonsils, and spleen
Salmonella culture SALM
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
(3) Virus FA
Porcine Rotavirus, PRRSV, Porcine circovirus-2
VIRFA
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Virus Isolation VI
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Yersinia culture YERS
  • +/- Intestinal swab placed in anaerobic transport media
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)

Prices

$459.30 (ante mortem samples)
$747.50 (full set of necropsy samples)

Notes

* If Brachyspira culture is positive, Brachyspira PCR can be used to further characterize the isolate. May want to consider the PED/TGE combination PCR. May also consider Cryptosporidium ELISA on piglets 1-6 months old (test performed on fresh feces). May want to consider Clostridium perfringens genotyping PCR for identification of the genes for specific toxins if Clostridium perfringens is isolated. If E. coli is isolated, consider Escherichia coli genotyping PCR to further identify which genes for which adhesins and toxins are present.

Ship chilled and keep frozen samples frozen except for Port-a-cul™ or Anaerobic Systems ATM and Parapak® which should be kept at room temperature. Protect fixed tissue from freezing.

Porcine Multisystemic Disease Diagnostic Plan

Please note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia, SIV, and PED. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • +/- Intestinal swab placed in anaerobic transport media
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Joint fluid in red top tube
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Alpha Coronavirus (TGEV) PCR ACOR
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Anaerobic bacterial culture ANAER
  • +/- Intestinal swab placed in anaerobic transport media
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Tied off loop of bowel with contents (frozen)
Brachyspira culture* RBRACH
  • Colon or cecum (preferred for Brachyspira)
  • Rectal swab
  • Tied off loop of bowel with contents (fresh)
Clostridium difficile Toxins (A/B) CLDT
  • Tied off loop of bowel with contents (frozen)
Clostridium perfringens Enterotoxin CLPT
  • Tied off loop of bowel with contents (frozen)
Cytology Smear Exam CYSMRE
  • (2) air dried slides of fluid
  • EDTA whole blood in lavender top tube
Fecal Floatation FLOAT
  • Fresh feces or colon contents in leak-proof container (10 g)
Histopathology HISTO
  • Full set of formalin fixed tissues (including multiple sections of intestinal tract)
Lawsonia PCR LAWPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Mycoplasma Culture MYCOPL
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Fresh tissue: lung
  • Joint fluid in red top tube
Mycoplasma hyorhinis PCR RMHYORPCR
  • Joint fluid in red top tube
  • Joint tissue such as synovium
Mycoplasma hyosynoviae PCR RMHYOSPCR
  • Joint fluid in red top tube
  • Joint tissue such as synovium
Porcine Circovirus type 2 PCR* RCIRCPCR
  • Fresh tissue: lung
Porcine Cytomegalovirus PCR* RPCYTPCR
  • Nasal turbinates or swab of turbinates in red top tube with 0.5ml sterile saline
Porcine Delta Coronavirus PCR* RPDCPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Epidemic Diarrhea Virus (PEDV) PCR* RPEDPCR
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
Pseudorabies PCR RPRVPCR
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
Salmonella culture SALM
  • +/- Intestinal swab placed in anaerobic transport media
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Swine Influenza Virus PCR SIPCR
  • Fresh tissue: lung
(6) Virus FA
Porcine circovirus-2, Porcine Reproductive and Respiratory Syndrome, Porcine Respiratory Coronavirus, Swine Influenza virus, Porcine Rotavirus, Porcine Parvovirus
VIRFA
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)
Virus Isolation VI
  • Fresh tissue: brain, lung, kidney, liver, heart, spleen, lymph nodes, tonsils, intestine
  • Tied off loop of bowel with contents (fresh)
  • Tied off loop of bowel with contents (frozen)

Prices

$1,201.75

Notes

Ship chilled and keep frozen samples frozen except for Port-a-cul™ and anaerobic transport systems atm which should be kept at room temperature. Protect fixed tissue from freezing.

If toxins are suspected a full toxicology set of tissues should be collected

Porcine Neurologic Diagnostic Plan

Please strongly consider rabies testing at your state health department.

Please note that Classical Swine Fever should also be considered if the signs are appropriate. If so, the proper state health officials should be notified immediately in order to initiate testing.

Please also note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia and SIV. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (1) swab inoculated with CSF
  • (1) swab of meninges prior to contamination and placed in aerobic transport media (Amies with charcoal)
  • CSF sample taken using sterile technique and placed in sterile red top tube
Field Necropsy Surcharge HISTONIB
  • Full set of fixed tissues placed in formalin, including multiple sections of brain and spinal cord.
Histopathology HISTO
  • Full set of fixed tissues placed in formalin, including multiple sections of brain and spinal cord.
Lead (blood) BDLEAD
  • EDTA or Heparin whole blood (lavender or green top tube) or fresh liver in sterile container
  • Fresh liver in sterile container
Lead (tissue) NCOX
  • EDTA or Heparin whole blood (lavender or green top tube) or fresh liver in sterile container
  • Fresh liver in sterile container
Listeria Culture LISTCUL
  • Fresh brain (preferably brainstem and cerebellum) placed in sterile container
Pseudorabies PCR RPRVPCR
  • Fresh brain (preferably brainstem and cerebellum) placed in sterile container
Salmonella culture SALM
  • (1) swab inoculated with CSF
  • (1) swab of meninges prior to contamination and placed in aerobic transport media (Amies with charcoal)
  • CSF sample taken using sterile technique and placed in sterile red top tube
Virus FA
Porcine Reproductive and Respiratory Syndrome, Porcine circovirus-2, Hemagglutination Encephalitis Virus (HEV)
VIRFA
  • Fresh brain (preferably brainstem and cerebellum) placed in sterile container
Virus Isolation VI
  • Fresh brain (preferably brainstem and cerebellum) placed in sterile container

Prices

$506.00 - $516.00

Notes

May also want to consider Cerebrospinal Fluid Cytology which requires CSF in EDTA tube (LTT) and (2) air dried slides of CSF. Protect slides from formalin exposure. If salt toxicity is a possibility, consider a mineral panel on fresh brain.

May be useful to check selenium level on an EDTA whole blood sample.

If whole head is submitted, a rabies brain extraction fee will be applied. Please submit completed NYS rabies specimen history form and write out clear instructions on what additional diagnostic tests should be performed.

Ship chilled and protect fixed tissue from freezing. Please indicate if rabies testing is pending. See tissues for rabies diagnosis for photos of brain samples needed for rabies testing. If toxins are suspected a full toxicology set of tissues should be collected.

Porcine Respiratory Diagnostic Plan

PLEASE NOTE THAT ACCURATE DIAGNOSIS OF RESPIRATORY DISEASE IN PIGS USUALLY REQUIRES SUBMISSION OF TISSUES. Results of tests on nasal/pharyngeal swabs only, must be evaluated with consideration of clinical signs. Accurate diagnosis requires the detection of the agent(s) as wells as compatible histologic lesions from post mortem samples.

Please also note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia, SIV and PED. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Ante mortem

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in aerobic transport media (Amies without charcoal)
Alpha Coronavirus (PRCV) PCR ACOR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
Cytology Smear Exam CYSMRE
  • (2) air dried slides of fluid
  • EDTA whole blood in lavender top tube
Mycoplasma Culture MYCOPL
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in aerobic transport media (Amies without charcoal)
Mycoplasma hyopneumoniae PCR* RMHYOPPCR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Oral fluid
Porcine Circovirus type 2 PCR* RCIRCPCR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Oral fluid
Porcine Cytomegalovirus PCR* RPCYTPCR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • EDTA whole blood in lavender top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Oral fluid
  • Serum (High serum antibody titers to TGE virus in herds with no evidence of TGE diarrhea may be suggestive of PRCV)
Porcine Respiratory Coronavirus (TGEV) ELISA* RTGEPRCEL
  • Serum (High serum antibody titers to TGE virus in herds with no evidence of TGE diarrhea may be suggestive of PRCV)
Swine Influenza Virus PCR SIPCR
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Oral fluid
Virus FA
Porcine circovirus-2, Porcine Reproductive and Respiratory Syndrome, Porcine Respiratory Coronavirus, Swine Influenza virus
VIRFA
  • Bronchial alveolar lavage (BAL) fluid in red top tube
Virus Isolation VI
  • Bronchial alveolar lavage (BAL) fluid in red top tube
  • Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline
  • Oral fluid

Necropsy

Tests Performed Test Code Samples Needed
Aerobic bacterial culture AER
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Section of fresh lung
Alpha Coronavirus (PRCV) PCR ACOR
  • Nasal swab in red top tube with 0.5ml sterile saline (PRCV is more readily detected from nasal swabs than from lung tissue)
  • Section of fresh lung
Field Necropsy Surcharge HISTONIB
  • Full set of tissues placed in formalin (include nasal turbinate for confirmation of porcine cytomegalovirus)
Histopathology HISTO
  • Full set of tissues placed in formalin (include nasal turbinate for confirmation of porcine cytomegalovirus)
Mycoplasma Culture MYCOPL
  • (1) swab inoculated with sample and placed in aerobic transport media (Amies without charcoal)
  • Section of fresh lung
Mycoplasma hyopneumoniae PCR* RMHYOPPCR
  • Section of fresh lung
Porcine Circovirus type 2 PCR* RCIRCPCR
  • Section of fresh lung
Porcine Cytomegalovirus PCR* RPCYTPCR
  • Brain (half fresh and half fixed in formalin)
  • Nasal swab in red top tube with 0.5ml sterile saline (PRCV is more readily detected from nasal swabs than from lung tissue)
  • Nasal turbinates
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Section of fresh lung
Swine Influenza Virus PCR SIPCR
  • Section of fresh lung
(4) Virus FA
Porcine circovirus-2, Porcine Reproductive and Respiratory Syndrome, Porcine Respiratory Coronavirus, Swine Influenza virus
VIRFA
  • Section of fresh lung
Virus Isolation VI
  • Section of fresh lung
  • Tonsil

Prices

$653.36 (ante mortem samples)
$586.36 (full set of necropsy samples)

Notes

Ship fresh tissue and swabs chilled and protect fixed tissue from freezing.

Porcine Sow Abortion Serology Diagnostic Plan

It is recommended to perform the porcine abortion fetal tissue diagnostic plan in addition to this plan. Please note that oral fluid makes a great sample for monitoring certain circulating pathogens in swine populations such as PRRSV, PCV2, Mycoplasma pneumonia and SIV. More information on collecting, shipping and testing oral fluid is available through Iowa State University.

Tests Performed Test Code Samples Needed
Leptospira MAT, 7 serovars LEPTOPNL
  • Maternal serum (separated)
Porcine Circovirus type 2 PCR* RCIRCPCR
  • Maternal serum (separated)
Porcine Parvovirus HI* RPPVHI
  • Maternal serum (separated)
Porcine Reproductive and Respiratory Syndrome ELISA RPRRSE
  • Maternal serum (separated)
Porcine Reproductive and Respiratory Syndrome PCR* RPRRS
  • Maternal serum (separated)
Selenium, whole blood SEL
  • Maternal EDTA whole blood in lavender top tube

Prices

$186.50 single
$230.00 paired (only 1 selenium, PCV-2 PCR and PRRSV PCR)

Notes

* For cases with mummified fetuses you may also want to do a Porcine Circovirus-2 IFA on sow’s serum.

Ship chilled.

Serum should be collected from additional sick cohort sows for PCR for PCV2 and PRRSV. When acute and convalescent samples are/will be collected, for most serology assays they should always be tested in parallel, and paired results interpreted. If acute samples are submitted alone please indicated if all tests should be performed. If second, convalescent samples are later submitted, tests on both samples will be run in parallel and fees for both samples will be charged. To avoid testing acute samples twice, hold and submit with convalescent samples. If convalescent samples will not be collected, or when infectious abortion is strongly suspected due to history, immediate testing of acute sample may be warranted.

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